Calculating alveolar capillary conductance and pulmonary capillary blood volume

Comparing the multiple-and single-inspired oxygen tension methods

Maile L. Ceridon, Kenneth C. Beck, Thomas P Olson, Jordan A. Bilezikian, Bruce David Johnson

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Key elements for determining alveolar-capillary membrane conductance (Dm) and pulmonary capillary blood volume (Vc) from the lung diffusing capacity (D L) for carbon monoxide (D LCO) or for nitric oxide (D Lno) are the reaction rate of carbon monoxide with hemoglobin (θ CO) and the Dm CO/D L NO relationship (α-ratio). Although a range of values have been reported, currently there is no consensus regarding these parameters. The study purpose was to define optimal parameters (θ CO, α-ratio) that would experimentally substantiate calculations of Dm and Vc from the single-inspired O 2 tension [inspired fraction of O 2 (Fi O2)] method relative to the multiple-Fi O2 method. Eight healthy men were studied at rest and during moderate exercise (80-W cycle). Dm and Vc were determined by the multiple-Fi O2 and single-Fi O2 methods (rebreathe technique) and were tabulated by applying previously reported θ CO equations (both methods) and by varying the a-ratio (single-Fi O2 method) from 1.90 to 2.50. Values were then compared between methods throughout the examined α-ratios. Dm and Vc were critically dependent on the applied θ CO equation. For the multiple-Fi O2 method, Dm was highly variable between θ CO equations (rest and exercise); the range of Vc was less widespread. For the single-Fi O2 method, the θ CO equation by Reeves and Park (1992) combined with an α-ratio between 2.08 and 2.26 gave values for Dm and Vc that most closely matched those from the multiple-Fi O2 method and were also physiologically plausible compared with predicted values. We conclude that the parameters used to calculate Dm and Vc values from the single-Fi O2 method (using D LCO and D LNO) can significantly influence results and should be evaluated within individual laboratories to obtain optimal values.

Original languageEnglish (US)
Pages (from-to)643-653
Number of pages11
JournalJournal of Applied Physiology
Volume109
Issue number3
DOIs
StatePublished - Sep 2010

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Carbon Monoxide
Blood Volume
Oxygen
Lung
Exercise
Lung Volume Measurements
Nitric Oxide
Hemoglobins
Membranes

Keywords

  • Carbon monoxide
  • Exercise
  • Gas exchange
  • Gas transfer
  • Nitric oxide

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Calculating alveolar capillary conductance and pulmonary capillary blood volume : Comparing the multiple-and single-inspired oxygen tension methods. / Ceridon, Maile L.; Beck, Kenneth C.; Olson, Thomas P; Bilezikian, Jordan A.; Johnson, Bruce David.

In: Journal of Applied Physiology, Vol. 109, No. 3, 09.2010, p. 643-653.

Research output: Contribution to journalArticle

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