Calcium-independent activation of skinned cardiac muscle by secophalloidin

Anna E. Bukatina, Lou J. Theodore, Kenneth B. Campbell, Gary C Sieck

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Thin filament regulation of muscle contraction is believed to be mediated by both Ca2+ and strongly bound myosin cross-bridges. We found that secophalloidin (SPH, 5-8 mM) activates cross-bridge cycling without Ca2+ causing isometric force comparable to that induced by Ca2+. At saturated [SPH], Ca2+ further increased force by 20%. SPH-induced force was reversible upon washing with a relaxing solution. However, there was more than 30% irreversible loss in subsequent Ca2+-activated force. We hypothesize that SPH activates muscle via strongly bound cross-bridges. SPH-activated contraction provides a new model for studying the role of Ca2+ and cross-bridges in muscle regulation.

Original languageEnglish (US)
Pages (from-to)201-204
Number of pages4
JournalFEBS Letters
Volume519
Issue number1-3
DOIs
StatePublished - May 22 2002

Fingerprint

Muscle
Myocardium
Chemical activation
Calcium
Muscles
Myosins
Muscle Contraction
Washing

Keywords

  • Activation
  • Cardiac muscle
  • Contraction
  • Regulation
  • Secophalloidin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Bukatina, A. E., Theodore, L. J., Campbell, K. B., & Sieck, G. C. (2002). Calcium-independent activation of skinned cardiac muscle by secophalloidin. FEBS Letters, 519(1-3), 201-204. https://doi.org/10.1016/S0014-5793(02)02766-7

Calcium-independent activation of skinned cardiac muscle by secophalloidin. / Bukatina, Anna E.; Theodore, Lou J.; Campbell, Kenneth B.; Sieck, Gary C.

In: FEBS Letters, Vol. 519, No. 1-3, 22.05.2002, p. 201-204.

Research output: Contribution to journalArticle

Bukatina, AE, Theodore, LJ, Campbell, KB & Sieck, GC 2002, 'Calcium-independent activation of skinned cardiac muscle by secophalloidin', FEBS Letters, vol. 519, no. 1-3, pp. 201-204. https://doi.org/10.1016/S0014-5793(02)02766-7
Bukatina, Anna E. ; Theodore, Lou J. ; Campbell, Kenneth B. ; Sieck, Gary C. / Calcium-independent activation of skinned cardiac muscle by secophalloidin. In: FEBS Letters. 2002 ; Vol. 519, No. 1-3. pp. 201-204.
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