Calbindin D28K interacts with Ran-binding protein M: Identification of interacting domains by NMR spectroscopy

Ward Lutz, Elena M. Frank, Theodore A. Craig, Richele Thompson, Ronald A. Venters, Doug Kojetin, John Cavanagh, Rajiv Kumar

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Calbindin D28K is an EF-hand containing protein that plays a vital role in neurological function. We now show that calcium-loaded calbindin D28K interacts with Ran-binding protein M, a protein known to play a role in microtubule function. Using NMR methods, we show that a peptide, LASIKNR, derived from Ran-binding protein M, interacts with several regions of the calcium-loaded protein including the amino terminus and two other regions that exhibit conformational exchange on the NMR timescale. We suggest that the interaction between calbindin D28K and Ran-binding protein M may be important in calbindin D28K function.

Original languageEnglish (US)
Pages (from-to)1186-1192
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume303
Issue number4
DOIs
StatePublished - Apr 18 2003

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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