Caffeine inhibits exercise-induced increase in tryptophan hydroxylase expression in dorsal and median raphe of Sprague-Dawley rats

Baek Vin Lim, Mi-Hyeon D Jang, Min Chul Shin, Hyun Bae Kim, Youn Jung Kim, Young Pyo Kim, Joo Ho Chung, Hong Kim, Mal Soon Shin, Sung Soo Kim, Ee Hwa Kim, Chang Ju Kim

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Effect of caffeine on the expression of tryptophan hydroxylase (TPH), rate limiting enzyme of serotonin synthesis, in dorsal and median raphe was investigated via immunohistochemistry. In exercise groups, Sprague-Dawley rats were put on treadmill running for 30 min per day for 6 consecutive days. On the seventh day, animals of control-with-caffeine group were injected subcutaneously with 4 mg/kg caffeine, while control-without-caffeine group were injected with 0.9% NaCl, sacrificed 2 h later. Exercise-with-caffeine group and exercise-without-caffeine group were injected with caffeine and NaCl, respectively; all-out time was determined 1 h after injection, and then sacrificed. Caffeine increased all-out time in exercised rats, and inhibited the exercise-induced elevation in TPH expression. The suppressive effect of caffeine on TPH expression in exercised rats can be suggested as one possible ergogenic mechanism of caffeine.

Original languageEnglish (US)
Pages (from-to)25-28
Number of pages4
JournalNeuroscience Letters
Volume308
Issue number1
DOIs
StatePublished - Jul 27 2001
Externally publishedYes

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Tryptophan Hydroxylase
Caffeine
Sprague Dawley Rats
Dorsal Raphe Nucleus
Running
Serotonin
Immunohistochemistry

Keywords

  • Caffeine
  • Dorsal raphe
  • Immunohistochemistry
  • Median raphe
  • Treadmill exercise
  • Tryptophan hydroxylase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Caffeine inhibits exercise-induced increase in tryptophan hydroxylase expression in dorsal and median raphe of Sprague-Dawley rats. / Lim, Baek Vin; Jang, Mi-Hyeon D; Shin, Min Chul; Kim, Hyun Bae; Kim, Youn Jung; Kim, Young Pyo; Chung, Joo Ho; Kim, Hong; Shin, Mal Soon; Kim, Sung Soo; Kim, Ee Hwa; Kim, Chang Ju.

In: Neuroscience Letters, Vol. 308, No. 1, 27.07.2001, p. 25-28.

Research output: Contribution to journalArticle

Lim, BV, Jang, M-HD, Shin, MC, Kim, HB, Kim, YJ, Kim, YP, Chung, JH, Kim, H, Shin, MS, Kim, SS, Kim, EH & Kim, CJ 2001, 'Caffeine inhibits exercise-induced increase in tryptophan hydroxylase expression in dorsal and median raphe of Sprague-Dawley rats', Neuroscience Letters, vol. 308, no. 1, pp. 25-28. https://doi.org/10.1016/S0304-3940(01)01980-2
Lim, Baek Vin ; Jang, Mi-Hyeon D ; Shin, Min Chul ; Kim, Hyun Bae ; Kim, Youn Jung ; Kim, Young Pyo ; Chung, Joo Ho ; Kim, Hong ; Shin, Mal Soon ; Kim, Sung Soo ; Kim, Ee Hwa ; Kim, Chang Ju. / Caffeine inhibits exercise-induced increase in tryptophan hydroxylase expression in dorsal and median raphe of Sprague-Dawley rats. In: Neuroscience Letters. 2001 ; Vol. 308, No. 1. pp. 25-28.
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