C-type natriuretic peptide: A selective cardiovascular peptide

A. L. Clavell, A. J. Stingo, C. M. Wei, D. M. Heublein, John C Jr. Burnett

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Studies were performed in three groups of anesthetized dogs to compare the structurally related peptides atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP). Group 1 (n = 5) and group 2 (n = 4) received intravenous infusions of CNP or ANP respectively at doses of 10 ng · kg-1 · min-1 and 100 ng · kg-1 · min-1. Group 3 (n = 5) received CNP intrarenally at doses of 1 ng · kg-1 · min-1 and 5 ng · kg-1 · min-1. Intravenous infusion of CNP resulted in a greater decrease in blood pressure when compared with ANP. This marked decrease in blood pressure observed with CNP was associated with a significantly smaller increase in guanosine 3',5'-cyclic monophosphate (cGMP). In contrast, neither intravenous nor intrarenal administration of CNP was associated with natriuresis as observed with ANP. The current study also demonstrates the presence of CNP immunoreactivity in canine plasma at low picomolar concentrations. Further characterization by gel permeation chromatography demonstrated that circulating CNP immunoreactivity corresponds to the 22-amino acid form of the peptide. In conclusion, this study demonstrates that CNP circulates in low picomolar concentrations and is potently vasoactive in vivo, suggesting a potential role in the regulation of vascular tone.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume264
Issue number2 33-2
StatePublished - 1993

Fingerprint

C-Type Natriuretic Peptide
Peptides
Atrial Natriuretic Factor
Intravenous Infusions
Blood Pressure
peptide A
Natriuresis
Cyclic GMP
Gel Chromatography
Blood Vessels
Canidae
Dogs

Keywords

  • guanosine 3',5'-cyclic monophosphate
  • mean arterial pressure
  • natriuretic peptides
  • renal sodium excretion

ASJC Scopus subject areas

  • Physiology

Cite this

C-type natriuretic peptide : A selective cardiovascular peptide. / Clavell, A. L.; Stingo, A. J.; Wei, C. M.; Heublein, D. M.; Burnett, John C Jr.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 264, No. 2 33-2, 1993.

Research output: Contribution to journalArticle

@article{e92af95558f24b5aa0eec6dc398803ca,
title = "C-type natriuretic peptide: A selective cardiovascular peptide",
abstract = "Studies were performed in three groups of anesthetized dogs to compare the structurally related peptides atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP). Group 1 (n = 5) and group 2 (n = 4) received intravenous infusions of CNP or ANP respectively at doses of 10 ng · kg-1 · min-1 and 100 ng · kg-1 · min-1. Group 3 (n = 5) received CNP intrarenally at doses of 1 ng · kg-1 · min-1 and 5 ng · kg-1 · min-1. Intravenous infusion of CNP resulted in a greater decrease in blood pressure when compared with ANP. This marked decrease in blood pressure observed with CNP was associated with a significantly smaller increase in guanosine 3',5'-cyclic monophosphate (cGMP). In contrast, neither intravenous nor intrarenal administration of CNP was associated with natriuresis as observed with ANP. The current study also demonstrates the presence of CNP immunoreactivity in canine plasma at low picomolar concentrations. Further characterization by gel permeation chromatography demonstrated that circulating CNP immunoreactivity corresponds to the 22-amino acid form of the peptide. In conclusion, this study demonstrates that CNP circulates in low picomolar concentrations and is potently vasoactive in vivo, suggesting a potential role in the regulation of vascular tone.",
keywords = "guanosine 3',5'-cyclic monophosphate, mean arterial pressure, natriuretic peptides, renal sodium excretion",
author = "Clavell, {A. L.} and Stingo, {A. J.} and Wei, {C. M.} and Heublein, {D. M.} and Burnett, {John C Jr.}",
year = "1993",
language = "English (US)",
volume = "264",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "2 33-2",

}

TY - JOUR

T1 - C-type natriuretic peptide

T2 - A selective cardiovascular peptide

AU - Clavell, A. L.

AU - Stingo, A. J.

AU - Wei, C. M.

AU - Heublein, D. M.

AU - Burnett, John C Jr.

PY - 1993

Y1 - 1993

N2 - Studies were performed in three groups of anesthetized dogs to compare the structurally related peptides atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP). Group 1 (n = 5) and group 2 (n = 4) received intravenous infusions of CNP or ANP respectively at doses of 10 ng · kg-1 · min-1 and 100 ng · kg-1 · min-1. Group 3 (n = 5) received CNP intrarenally at doses of 1 ng · kg-1 · min-1 and 5 ng · kg-1 · min-1. Intravenous infusion of CNP resulted in a greater decrease in blood pressure when compared with ANP. This marked decrease in blood pressure observed with CNP was associated with a significantly smaller increase in guanosine 3',5'-cyclic monophosphate (cGMP). In contrast, neither intravenous nor intrarenal administration of CNP was associated with natriuresis as observed with ANP. The current study also demonstrates the presence of CNP immunoreactivity in canine plasma at low picomolar concentrations. Further characterization by gel permeation chromatography demonstrated that circulating CNP immunoreactivity corresponds to the 22-amino acid form of the peptide. In conclusion, this study demonstrates that CNP circulates in low picomolar concentrations and is potently vasoactive in vivo, suggesting a potential role in the regulation of vascular tone.

AB - Studies were performed in three groups of anesthetized dogs to compare the structurally related peptides atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP). Group 1 (n = 5) and group 2 (n = 4) received intravenous infusions of CNP or ANP respectively at doses of 10 ng · kg-1 · min-1 and 100 ng · kg-1 · min-1. Group 3 (n = 5) received CNP intrarenally at doses of 1 ng · kg-1 · min-1 and 5 ng · kg-1 · min-1. Intravenous infusion of CNP resulted in a greater decrease in blood pressure when compared with ANP. This marked decrease in blood pressure observed with CNP was associated with a significantly smaller increase in guanosine 3',5'-cyclic monophosphate (cGMP). In contrast, neither intravenous nor intrarenal administration of CNP was associated with natriuresis as observed with ANP. The current study also demonstrates the presence of CNP immunoreactivity in canine plasma at low picomolar concentrations. Further characterization by gel permeation chromatography demonstrated that circulating CNP immunoreactivity corresponds to the 22-amino acid form of the peptide. In conclusion, this study demonstrates that CNP circulates in low picomolar concentrations and is potently vasoactive in vivo, suggesting a potential role in the regulation of vascular tone.

KW - guanosine 3',5'-cyclic monophosphate

KW - mean arterial pressure

KW - natriuretic peptides

KW - renal sodium excretion

UR - http://www.scopus.com/inward/record.url?scp=0027461436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027461436&partnerID=8YFLogxK

M3 - Article

C2 - 8383462

AN - SCOPUS:0027461436

VL - 264

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 2 33-2

ER -