Abstract
Studies were performed in three groups of anesthetized dogs to compare the structurally related peptides atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP). Group 1 (n = 5) and group 2 (n = 4) received intravenous infusions of CNP or ANP respectively at doses of 10 ng · kg-1 · min-1 and 100 ng · kg-1 · min-1. Group 3 (n = 5) received CNP intrarenally at doses of 1 ng · kg-1 · min-1 and 5 ng · kg-1 · min-1. Intravenous infusion of CNP resulted in a greater decrease in blood pressure when compared with ANP. This marked decrease in blood pressure observed with CNP was associated with a significantly smaller increase in guanosine 3',5'-cyclic monophosphate (cGMP). In contrast, neither intravenous nor intrarenal administration of CNP was associated with natriuresis as observed with ANP. The current study also demonstrates the presence of CNP immunoreactivity in canine plasma at low picomolar concentrations. Further characterization by gel permeation chromatography demonstrated that circulating CNP immunoreactivity corresponds to the 22-amino acid form of the peptide. In conclusion, this study demonstrates that CNP circulates in low picomolar concentrations and is potently vasoactive in vivo, suggesting a potential role in the regulation of vascular tone.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Regulatory Integrative and Comparative Physiology |
| Volume | 264 |
| Issue number | 2 33-2 |
| State | Published - 1993 |
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Keywords
- guanosine 3',5'-cyclic monophosphate
- mean arterial pressure
- natriuretic peptides
- renal sodium excretion
ASJC Scopus subject areas
- Physiology
Cite this
C-type natriuretic peptide : A selective cardiovascular peptide. / Clavell, A. L.; Stingo, A. J.; Wei, C. M.; Heublein, D. M.; Burnett, John C Jr.
In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 264, No. 2 33-2, 1993.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - C-type natriuretic peptide
T2 - A selective cardiovascular peptide
AU - Clavell, A. L.
AU - Stingo, A. J.
AU - Wei, C. M.
AU - Heublein, D. M.
AU - Burnett, John C Jr.
PY - 1993
Y1 - 1993
N2 - Studies were performed in three groups of anesthetized dogs to compare the structurally related peptides atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP). Group 1 (n = 5) and group 2 (n = 4) received intravenous infusions of CNP or ANP respectively at doses of 10 ng · kg-1 · min-1 and 100 ng · kg-1 · min-1. Group 3 (n = 5) received CNP intrarenally at doses of 1 ng · kg-1 · min-1 and 5 ng · kg-1 · min-1. Intravenous infusion of CNP resulted in a greater decrease in blood pressure when compared with ANP. This marked decrease in blood pressure observed with CNP was associated with a significantly smaller increase in guanosine 3',5'-cyclic monophosphate (cGMP). In contrast, neither intravenous nor intrarenal administration of CNP was associated with natriuresis as observed with ANP. The current study also demonstrates the presence of CNP immunoreactivity in canine plasma at low picomolar concentrations. Further characterization by gel permeation chromatography demonstrated that circulating CNP immunoreactivity corresponds to the 22-amino acid form of the peptide. In conclusion, this study demonstrates that CNP circulates in low picomolar concentrations and is potently vasoactive in vivo, suggesting a potential role in the regulation of vascular tone.
AB - Studies were performed in three groups of anesthetized dogs to compare the structurally related peptides atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP). Group 1 (n = 5) and group 2 (n = 4) received intravenous infusions of CNP or ANP respectively at doses of 10 ng · kg-1 · min-1 and 100 ng · kg-1 · min-1. Group 3 (n = 5) received CNP intrarenally at doses of 1 ng · kg-1 · min-1 and 5 ng · kg-1 · min-1. Intravenous infusion of CNP resulted in a greater decrease in blood pressure when compared with ANP. This marked decrease in blood pressure observed with CNP was associated with a significantly smaller increase in guanosine 3',5'-cyclic monophosphate (cGMP). In contrast, neither intravenous nor intrarenal administration of CNP was associated with natriuresis as observed with ANP. The current study also demonstrates the presence of CNP immunoreactivity in canine plasma at low picomolar concentrations. Further characterization by gel permeation chromatography demonstrated that circulating CNP immunoreactivity corresponds to the 22-amino acid form of the peptide. In conclusion, this study demonstrates that CNP circulates in low picomolar concentrations and is potently vasoactive in vivo, suggesting a potential role in the regulation of vascular tone.
KW - guanosine 3',5'-cyclic monophosphate
KW - mean arterial pressure
KW - natriuretic peptides
KW - renal sodium excretion
UR - http://www.scopus.com/inward/record.url?scp=0027461436&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027461436&partnerID=8YFLogxK
M3 - Article
C2 - 8383462
AN - SCOPUS:0027461436
VL - 264
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 2 33-2
ER -