C-reactive protein relaxes human vessels in vitro

Leonid Sternik, Saquib Samee, Hartzell V Schaff, Kenton J. Zehr, Lilach O Lerman, David Holmes, Joerg Herrmann, Amir Lerman

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Objective - C-reactive protein (CRP) is a sensitive marker of inflammation and a prognostic marker in cardiovascular disease. Evidence suggests direct biological activities of CRP within the vascular wall. The study was designed to examine the vasoreactive effects of CRP. Methods and Results - Human internal mammary artery rings were obtained during cardiovascular bypass surgery and suspended in an organ bath chamber. The rings were precontracted with endothelin-1, and response to cumulative concentrations of CRP was obtained. Experiments were repeated after initial incubation with 20, 40, and 60 mmol/L KCl, the potassium channel blockers BaCl, tetraethylammonium chloride, and glibenclamide, and the NO synthase inhibitor N-monomethyl-L-arginine and also after removal of the endothelium. CRP caused dose-dependent relaxation of human internal mammary artery rings, which was not affected by preincubation with N-monomethyl-L-arginine or removal of the endothelium. Maximum relaxation response to CRP (79.5±10%) was attenuated by KCl (2.5±11.5%, P<0.001), BaCl (24.5±7.5%, P<0.001), and tetraethylammonium chloride (34.9±8.25%, P<0.01) but not by glibenclamide. Conclusions - The present study demonstrates that CRP exerts an endothelium-independent vasorelaxing effect via potassium channels. Thus, the study suggests a role of CRP in the regulation of vascular tone.

Original languageEnglish (US)
Pages (from-to)1865-1868
Number of pages4
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume22
Issue number11
DOIs
StatePublished - Nov 1 2002

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C-Reactive Protein
Endothelium
Tetraethylammonium
Mammary Arteries
Glyburide
Blood Vessels
Arginine
Potassium Channel Blockers
In Vitro Techniques
Potassium Channels
Endothelin-1
Baths
Nitric Oxide Synthase
Cardiovascular Diseases
Inflammation

Keywords

  • Atherosclerosis
  • C-reactive protein
  • Inflammation
  • Potassium channels
  • Vasorelaxation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

C-reactive protein relaxes human vessels in vitro. / Sternik, Leonid; Samee, Saquib; Schaff, Hartzell V; Zehr, Kenton J.; Lerman, Lilach O; Holmes, David; Herrmann, Joerg; Lerman, Amir.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 22, No. 11, 01.11.2002, p. 1865-1868.

Research output: Contribution to journalArticle

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N2 - Objective - C-reactive protein (CRP) is a sensitive marker of inflammation and a prognostic marker in cardiovascular disease. Evidence suggests direct biological activities of CRP within the vascular wall. The study was designed to examine the vasoreactive effects of CRP. Methods and Results - Human internal mammary artery rings were obtained during cardiovascular bypass surgery and suspended in an organ bath chamber. The rings were precontracted with endothelin-1, and response to cumulative concentrations of CRP was obtained. Experiments were repeated after initial incubation with 20, 40, and 60 mmol/L KCl, the potassium channel blockers BaCl, tetraethylammonium chloride, and glibenclamide, and the NO synthase inhibitor N-monomethyl-L-arginine and also after removal of the endothelium. CRP caused dose-dependent relaxation of human internal mammary artery rings, which was not affected by preincubation with N-monomethyl-L-arginine or removal of the endothelium. Maximum relaxation response to CRP (79.5±10%) was attenuated by KCl (2.5±11.5%, P<0.001), BaCl (24.5±7.5%, P<0.001), and tetraethylammonium chloride (34.9±8.25%, P<0.01) but not by glibenclamide. Conclusions - The present study demonstrates that CRP exerts an endothelium-independent vasorelaxing effect via potassium channels. Thus, the study suggests a role of CRP in the regulation of vascular tone.

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