C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization

A. Rasim Barutcu, Andrew J. Fritz, Sayyed K. Zaidi, Andre J van Wijnen, Jane B. Lian, Janet L. Stein, Jeffrey A. Nickerson, Anthony N. Imbalzano, Gary S. Stein

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Three-dimensional organization of the chromatin has important roles in transcription, replication, DNA repair, and pathologic events such as translocations. There are two fundamental ways to study higher-order chromatin organization: microscopic and molecular approaches. In this review, we briefly introduce the molecular approaches, focusing on chromosome conformation capture or "3C" technology and its derivatives, which can be used to probe chromatin folding at resolutions beyond that provided by microscopy techniques. We further discuss the different types of data generated by the 3C-based methods and how they can be used to answer distinct biological questions.

Original languageEnglish (US)
Pages (from-to)31-35
Number of pages5
JournalJournal of Cellular Physiology
Volume231
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Chromosomes
Chromatin
Conformations
Genes
Genome
Transcription
DNA Repair
Microscopy
Microscopic examination
Repair
Technology
Derivatives
DNA

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Barutcu, A. R., Fritz, A. J., Zaidi, S. K., van Wijnen, A. J., Lian, J. B., Stein, J. L., ... Stein, G. S. (2016). C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization. Journal of Cellular Physiology, 231(1), 31-35. https://doi.org/10.1002/jcp.25062

C-ing the Genome : A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization. / Barutcu, A. Rasim; Fritz, Andrew J.; Zaidi, Sayyed K.; van Wijnen, Andre J; Lian, Jane B.; Stein, Janet L.; Nickerson, Jeffrey A.; Imbalzano, Anthony N.; Stein, Gary S.

In: Journal of Cellular Physiology, Vol. 231, No. 1, 01.01.2016, p. 31-35.

Research output: Contribution to journalArticle

Barutcu, AR, Fritz, AJ, Zaidi, SK, van Wijnen, AJ, Lian, JB, Stein, JL, Nickerson, JA, Imbalzano, AN & Stein, GS 2016, 'C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization', Journal of Cellular Physiology, vol. 231, no. 1, pp. 31-35. https://doi.org/10.1002/jcp.25062
Barutcu, A. Rasim ; Fritz, Andrew J. ; Zaidi, Sayyed K. ; van Wijnen, Andre J ; Lian, Jane B. ; Stein, Janet L. ; Nickerson, Jeffrey A. ; Imbalzano, Anthony N. ; Stein, Gary S. / C-ing the Genome : A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization. In: Journal of Cellular Physiology. 2016 ; Vol. 231, No. 1. pp. 31-35.
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