Butyrylcholinesterase in Human Brain and Acetylcholinesterase in Human Plasma: Trace Enzymes Measured by Two‐Site Immunoassay

Stephen Brimijoin, Pamela Hammond

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Abstract: Enzyme‐linked immunosorbent assays for acetylcholinesterase (AChE) and for butyrylcholinesterase (BuChE) were markedly more specific than conventional assays using selective enzyme inhibitors. The new assays were used with blood and brain samples containing traces of one enzyme dominated by large amounts of the other. The results showed that human plasma does contain AChE (8 ng/ml), even though its major cholinesterase is BuChE (3,300 ng/ml). BuChE immunoreactivity was not detected in human red blood cells but occurred in all brain regions. The cerebellum was the richest region tested (540 ng of BuChE/g of tissue), whereas the cerebral cortex was the poorest (240 ng of BuChE/g). However, because of the small local AChE content (99 ng/g), BuChE was the major cortical cholinesterase. The picture was reversed in the putamen, where BuChE immunoreactivity (340 ng/g) was far outweighed by that of AChE (6,100 ng/g).

Original languageEnglish (US)
Pages (from-to)1227-1231
Number of pages5
JournalJournal of neurochemistry
Volume51
Issue number4
DOIs
StatePublished - Oct 1988

Keywords

  • Acetylcholinesterase
  • Butyrylcholinesterase
  • Enzyme‐linked immunosorbent assay
  • Human brain
  • Human red blood cells
  • Plasma cholinesterase
  • ‐Human plasma

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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