TY - JOUR
T1 - Butyrylcholinesterase in Human Brain and Acetylcholinesterase in Human Plasma
T2 - Trace Enzymes Measured by Two‐Site Immunoassay
AU - Brimijoin, Stephen
AU - Hammond, Pamela
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1988/10
Y1 - 1988/10
N2 - Abstract: Enzyme‐linked immunosorbent assays for acetylcholinesterase (AChE) and for butyrylcholinesterase (BuChE) were markedly more specific than conventional assays using selective enzyme inhibitors. The new assays were used with blood and brain samples containing traces of one enzyme dominated by large amounts of the other. The results showed that human plasma does contain AChE (8 ng/ml), even though its major cholinesterase is BuChE (3,300 ng/ml). BuChE immunoreactivity was not detected in human red blood cells but occurred in all brain regions. The cerebellum was the richest region tested (540 ng of BuChE/g of tissue), whereas the cerebral cortex was the poorest (240 ng of BuChE/g). However, because of the small local AChE content (99 ng/g), BuChE was the major cortical cholinesterase. The picture was reversed in the putamen, where BuChE immunoreactivity (340 ng/g) was far outweighed by that of AChE (6,100 ng/g).
AB - Abstract: Enzyme‐linked immunosorbent assays for acetylcholinesterase (AChE) and for butyrylcholinesterase (BuChE) were markedly more specific than conventional assays using selective enzyme inhibitors. The new assays were used with blood and brain samples containing traces of one enzyme dominated by large amounts of the other. The results showed that human plasma does contain AChE (8 ng/ml), even though its major cholinesterase is BuChE (3,300 ng/ml). BuChE immunoreactivity was not detected in human red blood cells but occurred in all brain regions. The cerebellum was the richest region tested (540 ng of BuChE/g of tissue), whereas the cerebral cortex was the poorest (240 ng of BuChE/g). However, because of the small local AChE content (99 ng/g), BuChE was the major cortical cholinesterase. The picture was reversed in the putamen, where BuChE immunoreactivity (340 ng/g) was far outweighed by that of AChE (6,100 ng/g).
KW - Acetylcholinesterase
KW - Butyrylcholinesterase
KW - Enzyme‐linked immunosorbent assay
KW - Human brain
KW - Human red blood cells
KW - Plasma cholinesterase
KW - ‐Human plasma
UR - http://www.scopus.com/inward/record.url?scp=0023758710&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023758710&partnerID=8YFLogxK
U2 - 10.1111/j.1471-4159.1988.tb03091.x
DO - 10.1111/j.1471-4159.1988.tb03091.x
M3 - Article
C2 - 2901462
AN - SCOPUS:0023758710
SN - 0022-3042
VL - 51
SP - 1227
EP - 1231
JO - Journal of neurochemistry
JF - Journal of neurochemistry
IS - 4
ER -