Abstract
Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential "humanization" of the transmembrane domain (TMD) of the pig-tailed BST-2 (ptBST-2) protein resulted in a fluctuation in sensitivity to HIV-1 Vpu. Our results also show that the length of the TMD in human and ptBST-2 proteins is important for BST-2 restriction and susceptibility to Vpu. Taken together, our results emphasize the importance of tertiary structure in BST-2 antagonism and suggests that the HIV-1 Vpu transmembrane domain may have additional functions in vivo unrelated to BST-2 antagonism.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 312-321 |
| Number of pages | 10 |
| Journal | Virology |
| Volume | 406 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 1 2010 |
| Externally published | Yes |
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Keywords
- BST-2
- HIV-1
- Pig-tailed macaque
- Rhesus macaque
- SHIV
- Simian-human immunodeficiency virus
- Transmembrane domain
- Virus release
- Vpu
ASJC Scopus subject areas
- Virology
Cite this
BST-2 mediated restriction of simian-human immunodeficiency virus. / Schulze, Autumn; Lau, David; Mitchell, Richard S.; Hill, M. Sarah; Schmitt, Kimberly; Guatelli, John C.; Stephens, Edward B.
In: Virology, Vol. 406, No. 2, 01.10.2010, p. 312-321.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - BST-2 mediated restriction of simian-human immunodeficiency virus
AU - Schulze, Autumn
AU - Lau, David
AU - Mitchell, Richard S.
AU - Hill, M. Sarah
AU - Schmitt, Kimberly
AU - Guatelli, John C.
AU - Stephens, Edward B.
PY - 2010/10/1
Y1 - 2010/10/1
N2 - Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential "humanization" of the transmembrane domain (TMD) of the pig-tailed BST-2 (ptBST-2) protein resulted in a fluctuation in sensitivity to HIV-1 Vpu. Our results also show that the length of the TMD in human and ptBST-2 proteins is important for BST-2 restriction and susceptibility to Vpu. Taken together, our results emphasize the importance of tertiary structure in BST-2 antagonism and suggests that the HIV-1 Vpu transmembrane domain may have additional functions in vivo unrelated to BST-2 antagonism.
AB - Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential "humanization" of the transmembrane domain (TMD) of the pig-tailed BST-2 (ptBST-2) protein resulted in a fluctuation in sensitivity to HIV-1 Vpu. Our results also show that the length of the TMD in human and ptBST-2 proteins is important for BST-2 restriction and susceptibility to Vpu. Taken together, our results emphasize the importance of tertiary structure in BST-2 antagonism and suggests that the HIV-1 Vpu transmembrane domain may have additional functions in vivo unrelated to BST-2 antagonism.
KW - BST-2
KW - HIV-1
KW - Pig-tailed macaque
KW - Rhesus macaque
KW - SHIV
KW - Simian-human immunodeficiency virus
KW - Transmembrane domain
KW - Virus release
KW - Vpu
UR - http://www.scopus.com/inward/record.url?scp=78650041789&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650041789&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2010.07.021
DO - 10.1016/j.virol.2010.07.021
M3 - Article
VL - 406
SP - 312
EP - 321
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -