BST-2 mediated restriction of simian-human immunodeficiency virus

Autumn Schulze, David Lau, Richard S. Mitchell, M. Sarah Hill, Kimberly Schmitt, John C. Guatelli, Edward B. Stephens

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential "humanization" of the transmembrane domain (TMD) of the pig-tailed BST-2 (ptBST-2) protein resulted in a fluctuation in sensitivity to HIV-1 Vpu. Our results also show that the length of the TMD in human and ptBST-2 proteins is important for BST-2 restriction and susceptibility to Vpu. Taken together, our results emphasize the importance of tertiary structure in BST-2 antagonism and suggests that the HIV-1 Vpu transmembrane domain may have additional functions in vivo unrelated to BST-2 antagonism.

Original languageEnglish (US)
Pages (from-to)312-321
Number of pages10
JournalVirology
Volume406
Issue number2
DOIs
StatePublished - Oct 1 2010
Externally publishedYes

Fingerprint

Simian Immunodeficiency Virus
HIV-1
Swine
HIV
Virus Release
Proteins

Keywords

  • BST-2
  • HIV-1
  • Pig-tailed macaque
  • Rhesus macaque
  • SHIV
  • Simian-human immunodeficiency virus
  • Transmembrane domain
  • Virus release
  • Vpu

ASJC Scopus subject areas

  • Virology

Cite this

Schulze, A., Lau, D., Mitchell, R. S., Hill, M. S., Schmitt, K., Guatelli, J. C., & Stephens, E. B. (2010). BST-2 mediated restriction of simian-human immunodeficiency virus. Virology, 406(2), 312-321. https://doi.org/10.1016/j.virol.2010.07.021

BST-2 mediated restriction of simian-human immunodeficiency virus. / Schulze, Autumn; Lau, David; Mitchell, Richard S.; Hill, M. Sarah; Schmitt, Kimberly; Guatelli, John C.; Stephens, Edward B.

In: Virology, Vol. 406, No. 2, 01.10.2010, p. 312-321.

Research output: Contribution to journalArticle

Schulze, A, Lau, D, Mitchell, RS, Hill, MS, Schmitt, K, Guatelli, JC & Stephens, EB 2010, 'BST-2 mediated restriction of simian-human immunodeficiency virus', Virology, vol. 406, no. 2, pp. 312-321. https://doi.org/10.1016/j.virol.2010.07.021
Schulze A, Lau D, Mitchell RS, Hill MS, Schmitt K, Guatelli JC et al. BST-2 mediated restriction of simian-human immunodeficiency virus. Virology. 2010 Oct 1;406(2):312-321. https://doi.org/10.1016/j.virol.2010.07.021
Schulze, Autumn ; Lau, David ; Mitchell, Richard S. ; Hill, M. Sarah ; Schmitt, Kimberly ; Guatelli, John C. ; Stephens, Edward B. / BST-2 mediated restriction of simian-human immunodeficiency virus. In: Virology. 2010 ; Vol. 406, No. 2. pp. 312-321.
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