Abstract
Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential "humanization" of the transmembrane domain (TMD) of the pig-tailed BST-2 (ptBST-2) protein resulted in a fluctuation in sensitivity to HIV-1 Vpu. Our results also show that the length of the TMD in human and ptBST-2 proteins is important for BST-2 restriction and susceptibility to Vpu. Taken together, our results emphasize the importance of tertiary structure in BST-2 antagonism and suggests that the HIV-1 Vpu transmembrane domain may have additional functions in vivo unrelated to BST-2 antagonism.
Original language | English (US) |
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Pages (from-to) | 312-321 |
Number of pages | 10 |
Journal | Virology |
Volume | 406 |
Issue number | 2 |
DOIs | |
State | Published - Oct 2010 |
Keywords
- BST-2
- HIV-1
- Pig-tailed macaque
- Rhesus macaque
- SHIV
- Simian-human immunodeficiency virus
- Transmembrane domain
- Virus release
- Vpu
ASJC Scopus subject areas
- Virology