@article{8ab13b2e78ba4277925c49c6107f407a,
title = "BST-2 mediated restriction of simian-human immunodeficiency virus",
abstract = "Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential {"}humanization{"} of the transmembrane domain (TMD) of the pig-tailed BST-2 (ptBST-2) protein resulted in a fluctuation in sensitivity to HIV-1 Vpu. Our results also show that the length of the TMD in human and ptBST-2 proteins is important for BST-2 restriction and susceptibility to Vpu. Taken together, our results emphasize the importance of tertiary structure in BST-2 antagonism and suggests that the HIV-1 Vpu transmembrane domain may have additional functions in vivo unrelated to BST-2 antagonism.",
keywords = "BST-2, HIV-1, Pig-tailed macaque, Rhesus macaque, SHIV, Simian-human immunodeficiency virus, Transmembrane domain, Virus release, Vpu",
author = "Autumn Ruiz and David Lau and Mitchell, {Richard S.} and Hill, {M. Sarah} and Kimberly Schmitt and Guatelli, {John C.} and Stephens, {Edward B.}",
note = "Funding Information: The work reported here is supported by NIH grants AI51981 to E.B.S. and AI081688 to J.C.G. The following reagents were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH: Anti-Bst-2 (cat# 11722) from Drs. Klaus Strebel and Amy Andrew; and TZM-bl from Dr. John C. Kappes, Dr. Xiaoyun Wu and Tranzyme Inc.; and the pcDNAVphu (catalog #10076) from Drs. Stephen Bour and Klaus Strebel. The murine monoclonal antibody to BST-2 used for the flow cytometry was a gift from Chugai Pharmaceutical Co, Kanagawa, Japan. We also would like to thank members of the KUMC Biotechnology Support Facility and the Northwestern University Genomics Core Facility for their assistance with the oligonucleotide synthesis and sequence analysis.",
year = "2010",
month = oct,
doi = "10.1016/j.virol.2010.07.021",
language = "English (US)",
volume = "406",
pages = "312--321",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",
}