Bradykinin receptor-mediated cyclic GMP formation in a nerve cell population (murine neuroblastoma clone N1E-115)

R. M. Snider, E. Richelson

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

A clone of murine neuroblastoma (N1E-115) was shown to have functional receptors for the nonapeptide bradykinin. These receptors mediated a large, rapid (about 1 min to peak) and calcium-dependent increase in cyclic GMP. The median effective concentration (EC 50) averaged 1.4 nM. In addition, this event was inhibited by quinacrine, 5,8,11,14-eicosatetraynoic acid, and nordihydroguaiaretic acid, suggesting involvement of phospholipase A 2 with subsequent formation of lipoxygenase metabolites of arachidonic acid. [ 3H]Bradykinin binding to intact cells, investigated under conditions nearly identical to those used in the cyclic GMP assay, yielded binding sites with K(D)s of 0.83 pM, 1.0 nM, and 4.9 nM with respective B(max) values of 12, 160, and 250 fmol/10 6 cells. Apparently, the cyclic GMP response was associated with the binding site in which the K(D) = 1.0 nM. Peptide analogs of bradykinin stimulated cyclic GMP with EC 50s nearly identical to their respective K(D)s determined in binding assays with [ 3H]bradykinin, thus providing evidence for receptor specificity of this response. This finding of a biochemical response of bradykinin promises to make N1E-115 cells a convenient model system for study of neuronal bradykinin receptors.

Original languageEnglish (US)
Pages (from-to)1749-1754
Number of pages6
JournalJournal of Neurochemistry
Volume43
Issue number6
StatePublished - 1984

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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