TY - JOUR
T1 - Both systolic and diastolic dysfunction characterize nonischemic inhibition of myocardial energy metabolism
T2 - An experimental strain rate echocardiographic study
AU - Korinek, Josef
AU - Anagnostopoulos, Peter C.
AU - Pislaru, Cristina
AU - Dzeja, Petras
AU - Seward, James B.
AU - Terzic, Andre
AU - Belohlavek, Marek
PY - 2004/12
Y1 - 2004/12
N2 - Ischemia is primarily a metabolic event. However, regional functional changes can be affected by structural alterations. We developed an experimental model of sole myocardial energy metabolism inhibition and characterized the resulting regional dysfunction. In 12 pigs, we regionally inhibited creatine kinase (CK) and, consequently, myocyte high-energy phosphate transfer by intracoronary administration of iodoacetamide. Myocardial biopsies for CK activity and structural analyses and strain rate (SR) echocardiography scans were obtained at baseline and 60 minutes after iodoacetamide administration. Plasma levels of the CK isoenzyme MB and troponin I were assessed to determine possible myocardial damage. CK activity in the iodoacetamide-perfused myocardium decreased to 0.5% of the original value and was accompanied by a reduction in peak systolic SR (P <. 0001), end-systolic strain (P <. 0001), and peak SRs of myocardial early and late filling waves (P <. 0001). Microscopy showed contracture without sarcomere disruption. Plasma levels of CK isoenzyme MB and troponin I did not change. Regional inhibition of myocyte energetics leads to both systolic and diastolic dysfunction by SR echocardiography, but the presence of a residual phosphotransfer protects microstructural integrity.
AB - Ischemia is primarily a metabolic event. However, regional functional changes can be affected by structural alterations. We developed an experimental model of sole myocardial energy metabolism inhibition and characterized the resulting regional dysfunction. In 12 pigs, we regionally inhibited creatine kinase (CK) and, consequently, myocyte high-energy phosphate transfer by intracoronary administration of iodoacetamide. Myocardial biopsies for CK activity and structural analyses and strain rate (SR) echocardiography scans were obtained at baseline and 60 minutes after iodoacetamide administration. Plasma levels of the CK isoenzyme MB and troponin I were assessed to determine possible myocardial damage. CK activity in the iodoacetamide-perfused myocardium decreased to 0.5% of the original value and was accompanied by a reduction in peak systolic SR (P <. 0001), end-systolic strain (P <. 0001), and peak SRs of myocardial early and late filling waves (P <. 0001). Microscopy showed contracture without sarcomere disruption. Plasma levels of CK isoenzyme MB and troponin I did not change. Regional inhibition of myocyte energetics leads to both systolic and diastolic dysfunction by SR echocardiography, but the presence of a residual phosphotransfer protects microstructural integrity.
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U2 - 10.1016/j.echo.2004.08.015
DO - 10.1016/j.echo.2004.08.015
M3 - Article
C2 - 15562261
AN - SCOPUS:9644269126
SN - 0894-7317
VL - 17
SP - 1239
EP - 1244
JO - Journal of the American Society of Echocardiography
JF - Journal of the American Society of Echocardiography
IS - 12
ER -