Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States

Bobbi S. Pritt, Laurel B. Respicio-Kingry, Lynne M. Sloan, Martin E. Schriefer, Adam J. Replogle, Jenna Bjork, Gongping Liu, Luke C. Kingry, Paul S. Mead, David F. Neitzel, Elizabeth Schiffman, Diep K.Hoang Johnson, Jeffrey P. Davis, Susan M. Paskewitz, David Boxrud, Alecia Deedon, Xia Lee, Tracy K. Miller, Michelle A. Feist, Christopher R. StewardElitza S. Theel, Robin Patel, Cole L. Irish, Jeannine M. Petersen

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Lyme borreliosis (LB) is a multisystem disease caused by spirochetes in the Borrelia burgdorferi sensu lato (Bbsl) genospecies complex. We previously described a novel Bbsl genospecies (type strain MN14-1420T) that causes LB among patients with exposures to ticks in the upper midwestern USA. Patients infected with the novel Bbsl genospecies demonstrated higher levels of spirochetemia and somewhat differing clinical symptoms as compared with those infected with other Bbsl genospecies. The organism was detected from human specimens using PCR, microscopy, serology and culture. The taxonomic status was determined using an eighthousekeeping- gene (uvrA, rplB, recG, pyrG, pepX, clpX, clpA and nifS) multi-locus sequence analysis (MLSA) and comparison of 16S rRNA gene, flaB, rrf–rrl, ospC and oppA2 nucleotide sequences. Using a system threshold of 98.3% similarity for delineation of Bbsl genospecies by MLSA, we demonstrated that the novel species is a member of the Bbsl genospecies complex, most closely related to B. burgdorferi sensu stricto (94.7–94.9% similarity). This same species was identified in Ixodes scapularis ticks collected in Minnesota and Wisconsin. This novel species, Borrelia mayonii sp. nov, is formally described here. The type strain, MN14-1420, is available through the Deutsche Sammlung von Mikroorganismen und Zelkulturen GmbH (DSM 102811) and the American Type Culture Collection (ATCC BAA-2743).

Original languageEnglish (US)
Article number001455
Pages (from-to)4878-4880
Number of pages3
JournalInternational journal of systematic and evolutionary microbiology
Volume66
Issue number11
DOIs
StatePublished - Nov 2016

Keywords

  • Borrelia burgdorferi sensu lato
  • Lyme
  • Tick-borne
  • Vector-borne

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Fingerprint Dive into the research topics of 'Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States'. Together they form a unique fingerprint.

  • Cite this

    Pritt, B. S., Respicio-Kingry, L. B., Sloan, L. M., Schriefer, M. E., Replogle, A. J., Bjork, J., Liu, G., Kingry, L. C., Mead, P. S., Neitzel, D. F., Schiffman, E., Johnson, D. K. H., Davis, J. P., Paskewitz, S. M., Boxrud, D., Deedon, A., Lee, X., Miller, T. K., Feist, M. A., ... Petersen, J. M. (2016). Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States. International journal of systematic and evolutionary microbiology, 66(11), 4878-4880. [001455]. https://doi.org/10.1099/ijsem.0.001445