Bone marrow-derived heparan sulfate potentiates the osteogenic activity of bone morphogenetic protein-2 (BMP-2)

Diah S. Bramono, Sadasivam Murali, Bina Rai, Ling Ling, Wei Theng Poh, Zophia Xuehui Lim, Gary S. Stein, Victor Nurcombe, Andre J van Wijnen, Simon M. Cool

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Lowering the efficacious dose of bone morphogenetic protein-2 (BMP-2) for the repair of critical-sized bone defects is highly desirable, as supra-physiological amounts of BMP-2 have an increased risk of side effects and a greater economic burden for the healthcare system. To address this need, we explored the use of heparan sulfate (HS), a structural analog of heparin, to enhance BMP-2 activity. We demonstrate that HS isolated from a bone marrow stromal cell line (HS-5) and heparin each enhances BMP-2-induced osteogenesis in C2C12 myoblasts through increased ALP activity and osteocalcin mRNA expression. Commercially available HS variants from porcine kidney and bovine lung do not generate effects as great as HS5. Heparin and HS5 influence BMP-2 activity by (i) prolonging BMP-2 half-life, (ii) reducing interactions between BMP-2 with its antagonist noggin, and (iii) modulating BMP2 distribution on the cell surface. Importantly, long-term supplementation of HS5 but not heparin greatly enhances BMP-2-induced bone formation in vitro and in vivo. These results show that bone marrow-derived HS effectively supports bone formation, and suggest its applicability in bone repair by selectively facilitating the delivery and bioavailability of BMP-2.

Original languageEnglish (US)
Pages (from-to)954-964
Number of pages11
JournalBone
Volume50
Issue number4
DOIs
StatePublished - Apr 2012
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein 2
Heparitin Sulfate
Bone Marrow
Heparin
Osteogenesis
Bone and Bones
Myoblasts
Osteocalcin
Mesenchymal Stromal Cells
Biological Availability
Half-Life
Swine
Economics
Delivery of Health Care
Kidney
Cell Line
Lung
Messenger RNA

Keywords

  • Bone growth factor
  • Bone-forming
  • Glycosaminoglycan
  • Heparan sulfate
  • Heparin

ASJC Scopus subject areas

  • Physiology
  • Endocrinology, Diabetes and Metabolism
  • Histology

Cite this

Bramono, D. S., Murali, S., Rai, B., Ling, L., Poh, W. T., Lim, Z. X., ... Cool, S. M. (2012). Bone marrow-derived heparan sulfate potentiates the osteogenic activity of bone morphogenetic protein-2 (BMP-2). Bone, 50(4), 954-964. https://doi.org/10.1016/j.bone.2011.12.013

Bone marrow-derived heparan sulfate potentiates the osteogenic activity of bone morphogenetic protein-2 (BMP-2). / Bramono, Diah S.; Murali, Sadasivam; Rai, Bina; Ling, Ling; Poh, Wei Theng; Lim, Zophia Xuehui; Stein, Gary S.; Nurcombe, Victor; van Wijnen, Andre J; Cool, Simon M.

In: Bone, Vol. 50, No. 4, 04.2012, p. 954-964.

Research output: Contribution to journalArticle

Bramono, DS, Murali, S, Rai, B, Ling, L, Poh, WT, Lim, ZX, Stein, GS, Nurcombe, V, van Wijnen, AJ & Cool, SM 2012, 'Bone marrow-derived heparan sulfate potentiates the osteogenic activity of bone morphogenetic protein-2 (BMP-2)', Bone, vol. 50, no. 4, pp. 954-964. https://doi.org/10.1016/j.bone.2011.12.013
Bramono, Diah S. ; Murali, Sadasivam ; Rai, Bina ; Ling, Ling ; Poh, Wei Theng ; Lim, Zophia Xuehui ; Stein, Gary S. ; Nurcombe, Victor ; van Wijnen, Andre J ; Cool, Simon M. / Bone marrow-derived heparan sulfate potentiates the osteogenic activity of bone morphogenetic protein-2 (BMP-2). In: Bone. 2012 ; Vol. 50, No. 4. pp. 954-964.
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