A novel bone biopsy technique was used to generate a robust stromal cell system to study how stroma modulates CLL B-cell apoptosis and how the leukemic cell-stromal interaction influences secretion of vascular factors. Marrow stromal elements (MSE) rescued CLL B-cells from both spontaneous and drug induced apoptosis, partly due to soluble factors. When CLL B-cells were added to the MSE cultures, a dramatic increase in the secretion of basic fibroblast growth factor and decrease in the secretion of thrombospondin was observed. These results indicate the interaction between CLL B-cells and marrow stromal elements regulates angiogenic switching and may be linked to disease progression.
- Stromal cells
ASJC Scopus subject areas
- Cancer Research
Bone biopsy derived marrow stromal elements rescue chronic lymphocytic leukemia B-cells from spontaneous and drug induced cell death and facilitates an "angiogenic switch". / Kay, Neil Elliot; Shanafelt, Tait D.; Strege, Ann K.; Lee, Yean K.; Bone, Nancy D.; Raza, Azra.In: Leukemia Research, Vol. 31, No. 7, 07.2007, p. 899-906.
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