Bone biopsy derived marrow stromal elements rescue chronic lymphocytic leukemia B-cells from spontaneous and drug induced cell death and facilitates an "angiogenic switch"

Neil E. Kay, Tait D. Shanafelt, Ann K. Strege, Yean K. Lee, Nancy D. Bone, Azra Raza

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

A novel bone biopsy technique was used to generate a robust stromal cell system to study how stroma modulates CLL B-cell apoptosis and how the leukemic cell-stromal interaction influences secretion of vascular factors. Marrow stromal elements (MSE) rescued CLL B-cells from both spontaneous and drug induced apoptosis, partly due to soluble factors. When CLL B-cells were added to the MSE cultures, a dramatic increase in the secretion of basic fibroblast growth factor and decrease in the secretion of thrombospondin was observed. These results indicate the interaction between CLL B-cells and marrow stromal elements regulates angiogenic switching and may be linked to disease progression.

Original languageEnglish (US)
Pages (from-to)899-906
Number of pages8
JournalLeukemia Research
Volume31
Issue number7
DOIs
StatePublished - Jul 1 2007

Keywords

  • Apoptosis
  • CLL
  • Marrow
  • Stromal cells
  • VEGF

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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