Blue eye disease porcine rubulavirus (PoRv) infects pig neurons and glial cells using sialo-glycoprotein as receptor

Maria L. Mendoza-Magaña, Diana V. Godoy-Martinez, Hugo Guerrero-Cazares, Alejandra Rodriguez-Peredo, Judith M. Dueñas-Jimenez, Sergio H. Dueñas-Jiménez, Mario A. Ramírez-Herrera

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Pig neural cells express glycoproteins with sialylated N-linked oligosaccharide chains (SNOC) which are used by the porcine rubulavirus (PoRv) as receptors. Pig neuronal or glial cell cultures were employed to investigate (a) whether PoRv infects such cells using a molecule expressing SNOC, and (b) the role of viral envelope glycoproteins in establishing the infection. Enriched neuronal or glial cell cultures were exposed to PoRv and infection was detected immunocytochemically. Neuronal cultures prepared from neonatal pigs were treated enzymatically to eliminate sialic acid or N-linked oligosaccharide chains. Primary neural cultures were exposed to anti-HN or anti-F preincubated with PoRv to study the role of the viral glycoproteins. In enriched cultures, PoRv infected neurons and glial cells, and sialic acid expressed in N-linked oligosaccharide chains appeared to play a central role in infection. It was concluded that HN and F viral glycoproteins are required to infect neurons and glial cells.

Original languageEnglish (US)
Pages (from-to)428-436
Number of pages9
JournalVeterinary Journal
Volume173
Issue number2
DOIs
StatePublished - Mar 1 2007

Keywords

  • Cell receptor
  • Glycosidase
  • Neuronal culture
  • Rubulavirus
  • Viral infection

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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