Blood stem cell collection: Factors influencing the recovery of granulocyte-macrophage colony forming cells

Kevin Imrie, A. Keith Stewart, R. Michael Crump, H. Miles Prince, Kathy Trip, Armand Keating

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We evaluated data from all blond cell (BC) collections performed in our institution between 1989 and 1995 to determine factors influencing the outcome of collection. One hundred and thirty-three collections were performed on 106 patients. Malignant diagnoses were: non-Hodgkins lymphoma (NHL) in 35%, multiple myeloma in 31%, breast cancer in 26%, and Hodgkin's disease in 8%. Collections were obtained routinely in myeloma and breast cancer and due to bone marrow involvement with malignancy or inaspirable bone marrow in lymphoma patients. Collections were obtained on a Cobe Spectra or Baxter-Fenwall CS3000+. Engraftment potential was determined by methylcellulose colony assay (CFU-GM), with a target of >10 x 104 CFU- GM/kg. Apheresis nucleated cell count correlated significantly, albeit weakly (r = 0.26), with CFU-GM with a cell count of >5 x 108/kg resulting in an adequate number of CFU-GM in 78% of patients. In univariant analysis outcome of collection was significantly influenced by the patients age (p = 0.01), malignant diagnosis (p < 0.001), reason for collection (p = 0.002), and the mobilization regimen (p = 0.01). The nature of the apheresis device used did not influence outcome. Only malignant diagnosis was significant (p < 0.001) in multivariate analysis. We conclude that the outcome of BC is most strongly influenced by patient factors such as malignant diagnosis. These factors must be considered when comparing the outcome of different mobilization regimens and when planning collection strategies.

Original languageEnglish (US)
Pages (from-to)379-383
Number of pages5
JournalCell Transplantation
Volume5
Issue number3
DOIs
StatePublished - Jan 1 1996

Keywords

  • Autologous transplant
  • Blood stem cells
  • Colony forming cells

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Fingerprint Dive into the research topics of 'Blood stem cell collection: Factors influencing the recovery of granulocyte-macrophage colony forming cells'. Together they form a unique fingerprint.

Cite this