Blood endothelial cells

Robert D. Simari, Rajiv Gulati, Robert P. Hebbel

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In 1963, in an attempt to define the source of endothelium on vascular grafts, Stump and colleagues suspended a Dacron patch within the lumen of a prosthetic vascular graft in the aorta of a juvenile pig (1). As early as 14 days following placement, islands of endothelial cells (ECs) were identified on the patch surface. Because the patch had been isolated from contact with both the prosthesis and native vascular tissue, these findings implicated circulating blood as the source of ECs. These observations were not actively pursued from an experimental or clinical context until recently. This original observation of a vascular source of endothelium has been corroborated in chimeric transplantation models that have enabled the discrimination of host-and donor derived cells by genetic markers. These studies have revealed bone marrow–derived circulating progenitors to contribute to both endothelial and intimal smooth muscle cell formation in multiple models of vascular injury as reviewed by Sata (2). Moreover, treatment with 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase inhibitors (statins) appears to accelerate the incorporation of bone marrow–derived ECs following arterial denudation in rodent models (3,4). These studies confirm the presence of cells with either an endothelial phenotype or endothelial potential within human blood. Furthermore, strategies have been developed to utilize these cells for the prevention and treatment of vascular disease. In this chapter, identification, classification, and potential translational uses of these cells will be discussed. CIRCULATING ENDOTHELIAL CELLS Definition and Phenotype The blood of normal individuals contains circulating ECs (CECs) as well as monocytic cells with the potential to develop endothelial features in culture (culture-modified mononuclear cells – CMMCs) and progenitors capable of differentiation into ECs (so-called true endothelial progenitor cells [EPCs]).

Original languageEnglish (US)
Title of host publicationEndothelial Biomedicine
PublisherCambridge University Press
Pages1612-1620
Number of pages9
Volume9780521853767
ISBN (Print)9780511546198, 0521853761, 9780521853767
DOIs
StatePublished - Jan 1 2007

Fingerprint

Endothelial cells
Blood Cells
Blood
Endothelial Cells
Vascular Endothelium
Grafts
Tunica Intima
Bone
Blood Vessel Prosthesis
Transplants
Phenotype
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Bone and Bones
Polyethylene Terephthalates
Vascular System Injuries
Genetic Markers
Vascular Diseases
Smooth Muscle Myocytes
Blood Vessels
Aorta

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Simari, R. D., Gulati, R., & Hebbel, R. P. (2007). Blood endothelial cells. In Endothelial Biomedicine (Vol. 9780521853767, pp. 1612-1620). Cambridge University Press. https://doi.org/10.1017/CBO9780511546198.175

Blood endothelial cells. / Simari, Robert D.; Gulati, Rajiv; Hebbel, Robert P.

Endothelial Biomedicine. Vol. 9780521853767 Cambridge University Press, 2007. p. 1612-1620.

Research output: Chapter in Book/Report/Conference proceedingChapter

Simari, RD, Gulati, R & Hebbel, RP 2007, Blood endothelial cells. in Endothelial Biomedicine. vol. 9780521853767, Cambridge University Press, pp. 1612-1620. https://doi.org/10.1017/CBO9780511546198.175
Simari RD, Gulati R, Hebbel RP. Blood endothelial cells. In Endothelial Biomedicine. Vol. 9780521853767. Cambridge University Press. 2007. p. 1612-1620 https://doi.org/10.1017/CBO9780511546198.175
Simari, Robert D. ; Gulati, Rajiv ; Hebbel, Robert P. / Blood endothelial cells. Endothelial Biomedicine. Vol. 9780521853767 Cambridge University Press, 2007. pp. 1612-1620
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