Abstract
In 1963, in an attempt to define the source of endothelium on vascular grafts, Stump and colleagues suspended a Dacron patch within the lumen of a prosthetic vascular graft in the aorta of a juvenile pig (1). As early as 14 days following placement, islands of endothelial cells (ECs) were identified on the patch surface. Because the patch had been isolated from contact with both the prosthesis and native vascular tissue, these findings implicated circulating blood as the source of ECs. These observations were not actively pursued from an experimental or clinical context until recently. This original observation of a vascular source of endothelium has been corroborated in chimeric transplantation models that have enabled the discrimination of host-and donor derived cells by genetic markers. These studies have revealed bone marrow–derived circulating progenitors to contribute to both endothelial and intimal smooth muscle cell formation in multiple models of vascular injury as reviewed by Sata (2). Moreover, treatment with 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase inhibitors (statins) appears to accelerate the incorporation of bone marrow–derived ECs following arterial denudation in rodent models (3,4). These studies confirm the presence of cells with either an endothelial phenotype or endothelial potential within human blood. Furthermore, strategies have been developed to utilize these cells for the prevention and treatment of vascular disease. In this chapter, identification, classification, and potential translational uses of these cells will be discussed. CIRCULATING ENDOTHELIAL CELLS Definition and Phenotype The blood of normal individuals contains circulating ECs (CECs) as well as monocytic cells with the potential to develop endothelial features in culture (culture-modified mononuclear cells – CMMCs) and progenitors capable of differentiation into ECs (so-called true endothelial progenitor cells [EPCs]).
| Original language | English (US) |
|---|---|
| Title of host publication | Endothelial Biomedicine |
| Publisher | Cambridge University Press |
| Pages | 1612-1620 |
| Number of pages | 9 |
| Volume | 9780521853767 |
| ISBN (Print) | 9780511546198, 0521853761, 9780521853767 |
| DOIs | |
| State | Published - Jan 1 2007 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Blood endothelial cells. / Simari, Robert D.; Gulati, Rajiv; Hebbel, Robert P.
Endothelial Biomedicine. Vol. 9780521853767 Cambridge University Press, 2007. p. 1612-1620.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Blood endothelial cells
AU - Simari, Robert D.
AU - Gulati, Rajiv
AU - Hebbel, Robert P.
PY - 2007/1/1
Y1 - 2007/1/1
N2 - In 1963, in an attempt to define the source of endothelium on vascular grafts, Stump and colleagues suspended a Dacron patch within the lumen of a prosthetic vascular graft in the aorta of a juvenile pig (1). As early as 14 days following placement, islands of endothelial cells (ECs) were identified on the patch surface. Because the patch had been isolated from contact with both the prosthesis and native vascular tissue, these findings implicated circulating blood as the source of ECs. These observations were not actively pursued from an experimental or clinical context until recently. This original observation of a vascular source of endothelium has been corroborated in chimeric transplantation models that have enabled the discrimination of host-and donor derived cells by genetic markers. These studies have revealed bone marrow–derived circulating progenitors to contribute to both endothelial and intimal smooth muscle cell formation in multiple models of vascular injury as reviewed by Sata (2). Moreover, treatment with 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase inhibitors (statins) appears to accelerate the incorporation of bone marrow–derived ECs following arterial denudation in rodent models (3,4). These studies confirm the presence of cells with either an endothelial phenotype or endothelial potential within human blood. Furthermore, strategies have been developed to utilize these cells for the prevention and treatment of vascular disease. In this chapter, identification, classification, and potential translational uses of these cells will be discussed. CIRCULATING ENDOTHELIAL CELLS Definition and Phenotype The blood of normal individuals contains circulating ECs (CECs) as well as monocytic cells with the potential to develop endothelial features in culture (culture-modified mononuclear cells – CMMCs) and progenitors capable of differentiation into ECs (so-called true endothelial progenitor cells [EPCs]).
AB - In 1963, in an attempt to define the source of endothelium on vascular grafts, Stump and colleagues suspended a Dacron patch within the lumen of a prosthetic vascular graft in the aorta of a juvenile pig (1). As early as 14 days following placement, islands of endothelial cells (ECs) were identified on the patch surface. Because the patch had been isolated from contact with both the prosthesis and native vascular tissue, these findings implicated circulating blood as the source of ECs. These observations were not actively pursued from an experimental or clinical context until recently. This original observation of a vascular source of endothelium has been corroborated in chimeric transplantation models that have enabled the discrimination of host-and donor derived cells by genetic markers. These studies have revealed bone marrow–derived circulating progenitors to contribute to both endothelial and intimal smooth muscle cell formation in multiple models of vascular injury as reviewed by Sata (2). Moreover, treatment with 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase inhibitors (statins) appears to accelerate the incorporation of bone marrow–derived ECs following arterial denudation in rodent models (3,4). These studies confirm the presence of cells with either an endothelial phenotype or endothelial potential within human blood. Furthermore, strategies have been developed to utilize these cells for the prevention and treatment of vascular disease. In this chapter, identification, classification, and potential translational uses of these cells will be discussed. CIRCULATING ENDOTHELIAL CELLS Definition and Phenotype The blood of normal individuals contains circulating ECs (CECs) as well as monocytic cells with the potential to develop endothelial features in culture (culture-modified mononuclear cells – CMMCs) and progenitors capable of differentiation into ECs (so-called true endothelial progenitor cells [EPCs]).
UR - http://www.scopus.com/inward/record.url?scp=84928865483&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84928865483&partnerID=8YFLogxK
U2 - 10.1017/CBO9780511546198.175
DO - 10.1017/CBO9780511546198.175
M3 - Chapter
AN - SCOPUS:84928865483
SN - 9780511546198
SN - 0521853761
SN - 9780521853767
VL - 9780521853767
SP - 1612
EP - 1620
BT - Endothelial Biomedicine
PB - Cambridge University Press
ER -