Biphasic and regionally-restricted chemokine expression in the central nervous system in the Theiler's virus model of multiple sclerosis

Paul D. Murray, Kim Krivacic, Ann Chernosky, Tao Wei, Richard M. Ransohoff, Moses Rodriguez

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Intracerebral infection of susceptible strains of mice with Theiler's murine encephalomyelitis virus (TMEV) induces a biphasic disease characterized by acute polioencephalitis followed by chronic demyelination and viral persistence in the spinal cord white matter. There has been limited study of soluble mediators responsible for the initial recruitment of inflammatory cells into the gray matter, and the secondary influx into the white matter during infection with TMEV. We used sensitive and specific RT-PCR/dot blot hybridization assays to quantitate the relative levels of chemokine mRNA in the brains and spinal cords during the acute and chronic phases of TMEV infection in mice susceptible (B10.M, H-2(f)) and resistant (B10, H-2b) to virus-induced demyelination. TMEV infection resulted in robust expression of mRNA for IP-10, RANTES, and MCP-1, but not GRO-α, in brains and spinal cords in both strains of mice within 5 days. By day 21, virus was cleared, inflammation reduced, and expression of all three chemokines subsided to baseline levels in the brains and spinal cords of resistant mice, and the brains of susceptible mice. Chemokine expression was also reduced in the spinal cords of susceptible mice, corresponding to a shift in TMEV replication from the gray to the white matter. During the chronic, demyelinating phase of infection, there was a resurgence in IP-10, RANTES, and MCP-1 mRNA in spinal cords of susceptible B10.M mice. This study demonstrates the coordinated regulation and regionally restricted expression of chemokines in a biphasic disease of the central nervous system and provides greater understanding of the mechanism by which inflammation is established and maintained in the CNS.

Original languageEnglish (US)
JournalJournal of NeuroVirology
Volume6
Issue numberSUPPL. 1
StatePublished - 2000

Fingerprint

Theilovirus
Chemokines
Multiple Sclerosis
Central Nervous System
Spinal Cord
Chemokine CCL5
Brain
Demyelinating Diseases
Virus Diseases
Messenger RNA
Infection
Viruses
Inflammation
Central Nervous System Diseases
Acute Disease
Virus Replication
Polymerase Chain Reaction

Keywords

  • Chemoattractants
  • Demyelination
  • Encephalomyelitis
  • Mice
  • MS
  • Picornavirus
  • TMEV

ASJC Scopus subject areas

  • Virology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Murray, P. D., Krivacic, K., Chernosky, A., Wei, T., Ransohoff, R. M., & Rodriguez, M. (2000). Biphasic and regionally-restricted chemokine expression in the central nervous system in the Theiler's virus model of multiple sclerosis. Journal of NeuroVirology, 6(SUPPL. 1).

Biphasic and regionally-restricted chemokine expression in the central nervous system in the Theiler's virus model of multiple sclerosis. / Murray, Paul D.; Krivacic, Kim; Chernosky, Ann; Wei, Tao; Ransohoff, Richard M.; Rodriguez, Moses.

In: Journal of NeuroVirology, Vol. 6, No. SUPPL. 1, 2000.

Research output: Contribution to journalArticle

Murray, PD, Krivacic, K, Chernosky, A, Wei, T, Ransohoff, RM & Rodriguez, M 2000, 'Biphasic and regionally-restricted chemokine expression in the central nervous system in the Theiler's virus model of multiple sclerosis', Journal of NeuroVirology, vol. 6, no. SUPPL. 1.
Murray, Paul D. ; Krivacic, Kim ; Chernosky, Ann ; Wei, Tao ; Ransohoff, Richard M. ; Rodriguez, Moses. / Biphasic and regionally-restricted chemokine expression in the central nervous system in the Theiler's virus model of multiple sclerosis. In: Journal of NeuroVirology. 2000 ; Vol. 6, No. SUPPL. 1.
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