Biparental inheritance of γ-tubulin during human fertilization

Molecular reconstitution of functional zygotic centrosomes in inseminated human oocytes and in cell-free extracts nucleated by human sperm

Calvin Simerly, Sara S. Zoran, Chris Payne, Tanja Dominko, Peter Sutovsky, Christopher S. Navara, Jeffrey L Salisbury, Gerald Schatten

Research output: Contribution to journalArticle

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Abstract

Human sperm centrosome reconstitution and the parental contributions to the zygotic centrosome are examined in mammalian zygotes and after exposure of spermatozoa to Xenopus laevis cell-free extracts. The presence and inheritance of the conserved centrosomal constituents γ-tubulin, centrin, and MPM-2 (which detects phosphorylated epitopes) are traced, as is the sperm microtubule-nucleating capability on reconstituted centrosomes. γ-Tubulin is biparentally inherited in humans (maternal >> than paternal): Western blots detect the presence of paternal γ-tubulin. Recruitment of maternal γ- tubulin to the sperm centrosome occurs after sperm incorporation in vivo or exposure to cell-free extract, especially after sperm 'priming' induced by disulfide bond reduction. Centrin is found in the proximal sperm centrosomal region, demonstrates expected calcium sensitivity, but appears absent from the zygotic centrosome after sperm incorporation or exposure to extracts. Sperm centrosome phosphorylation is detected after exposure of primed sperm to egg extracts as well as during the early stages of sperm incorporation after fertilization. Finally, centrosome reconstitution in cell-free extracts permits sperm aster microtubule assembly in vitro. Collectively, these results support a model of a blended zygotic centrosome composed of maternal constituents attracted to an introduced paternal template after insemination.

Original languageEnglish (US)
Pages (from-to)2955-2969
Number of pages15
JournalMolecular Biology of the Cell
Volume10
Issue number9
StatePublished - Sep 1999

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Centrosome
Tubulin
Cell Extracts
Fertilization
Oocytes
Spermatozoa
Sulfamethoxazole Drug Combination Trimethoprim
Mothers
Microtubules
Insemination
Zygote
Xenopus laevis
Disulfides
Ovum
Epitopes
Western Blotting

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Biparental inheritance of γ-tubulin during human fertilization : Molecular reconstitution of functional zygotic centrosomes in inseminated human oocytes and in cell-free extracts nucleated by human sperm. / Simerly, Calvin; Zoran, Sara S.; Payne, Chris; Dominko, Tanja; Sutovsky, Peter; Navara, Christopher S.; Salisbury, Jeffrey L; Schatten, Gerald.

In: Molecular Biology of the Cell, Vol. 10, No. 9, 09.1999, p. 2955-2969.

Research output: Contribution to journalArticle

Simerly, Calvin ; Zoran, Sara S. ; Payne, Chris ; Dominko, Tanja ; Sutovsky, Peter ; Navara, Christopher S. ; Salisbury, Jeffrey L ; Schatten, Gerald. / Biparental inheritance of γ-tubulin during human fertilization : Molecular reconstitution of functional zygotic centrosomes in inseminated human oocytes and in cell-free extracts nucleated by human sperm. In: Molecular Biology of the Cell. 1999 ; Vol. 10, No. 9. pp. 2955-2969.
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