Biosynthesis of the 25-kDa protein in the macrogametes/zygotes of Plasmodium falciparum

Hella C.W. Fries; Marieke B.A.C. Lamers; Jan van Deursen; Thivi Ponnudurai; Joseph H.E.Th Meuwissen

doi:10.1016/0014-4894(90)90025-8

Biosynthesis of the 25-kDa protein in the macrogametes/zygotes of Plasmodium falciparum

Hella C.W. Fries, Marieke B.A.C. Lamers, Jan van Deursen, Thivi Ponnudurai, Joseph H.E.Th Meuwissen

Pediatric and Adolescent Medicine

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Synthesis of the 25-kDa protein in the early midgut stages of Plasmodium falciparum was studied, using metabolic inhibitors (colchicine and actinomycin D) and pulse-labeling experiments. Experiments with colchicine showed that, immediately after induction of macrogametogenesis, 25-kDa protein synthesis occurs in both fertilized and nonfertilized macrogametes. The amount of 25-kDa protein synthesized increased slowly during time. Experiments with actinomycin D revealed that the slow increase of synthesis may be dependent on de novo messenger RNA synthesis.

Original language	English (US)
Pages (from-to)	229-235
Number of pages	7
Journal	Experimental Parasitology
Volume	71
Issue number	2
DOIs	https://doi.org/10.1016/0014-4894(90)90025-8
State	Published - Aug 1990

Keywords

Actinomycin D
Colchicine
Plasmoduim falciparum
Pulse labeling

ASJC Scopus subject areas

Parasitology
Immunology
Infectious Diseases

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10.1016/0014-4894(90)90025-8

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title = "Biosynthesis of the 25-kDa protein in the macrogametes/zygotes of Plasmodium falciparum",

abstract = "Synthesis of the 25-kDa protein in the early midgut stages of Plasmodium falciparum was studied, using metabolic inhibitors (colchicine and actinomycin D) and pulse-labeling experiments. Experiments with colchicine showed that, immediately after induction of macrogametogenesis, 25-kDa protein synthesis occurs in both fertilized and nonfertilized macrogametes. The amount of 25-kDa protein synthesized increased slowly during time. Experiments with actinomycin D revealed that the slow increase of synthesis may be dependent on de novo messenger RNA synthesis.",

keywords = "Actinomycin D, Colchicine, Plasmoduim falciparum, Pulse labeling",

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AU - Fries, Hella C.W.

AU - Lamers, Marieke B.A.C.

AU - van Deursen, Jan

AU - Ponnudurai, Thivi

AU - Meuwissen, Joseph H.E.Th

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N2 - Synthesis of the 25-kDa protein in the early midgut stages of Plasmodium falciparum was studied, using metabolic inhibitors (colchicine and actinomycin D) and pulse-labeling experiments. Experiments with colchicine showed that, immediately after induction of macrogametogenesis, 25-kDa protein synthesis occurs in both fertilized and nonfertilized macrogametes. The amount of 25-kDa protein synthesized increased slowly during time. Experiments with actinomycin D revealed that the slow increase of synthesis may be dependent on de novo messenger RNA synthesis.

AB - Synthesis of the 25-kDa protein in the early midgut stages of Plasmodium falciparum was studied, using metabolic inhibitors (colchicine and actinomycin D) and pulse-labeling experiments. Experiments with colchicine showed that, immediately after induction of macrogametogenesis, 25-kDa protein synthesis occurs in both fertilized and nonfertilized macrogametes. The amount of 25-kDa protein synthesized increased slowly during time. Experiments with actinomycin D revealed that the slow increase of synthesis may be dependent on de novo messenger RNA synthesis.

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KW - Colchicine

KW - Plasmoduim falciparum

KW - Pulse labeling

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Biosynthesis of the 25-kDa protein in the macrogametes/zygotes of Plasmodium falciparum

Abstract

Keywords

ASJC Scopus subject areas

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