Biosynthesis of the 25-kDa protein in the macrogametes/zygotes of Plasmodium falciparum

Hella C.W. Fries, Marieke B.A.C. Lamers, Jan van Deursen, Thivi Ponnudurai, Joseph H.E.Th Meuwissen

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Synthesis of the 25-kDa protein in the early midgut stages of Plasmodium falciparum was studied, using metabolic inhibitors (colchicine and actinomycin D) and pulse-labeling experiments. Experiments with colchicine showed that, immediately after induction of macrogametogenesis, 25-kDa protein synthesis occurs in both fertilized and nonfertilized macrogametes. The amount of 25-kDa protein synthesized increased slowly during time. Experiments with actinomycin D revealed that the slow increase of synthesis may be dependent on de novo messenger RNA synthesis.

Original languageEnglish (US)
Pages (from-to)229-235
Number of pages7
JournalExperimental Parasitology
Volume71
Issue number2
DOIs
StatePublished - Aug 1990

Keywords

  • Actinomycin D
  • Colchicine
  • Plasmoduim falciparum
  • Pulse labeling

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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