Biosynthesis of the 25-kDa protein in the macrogametes/zygotes of Plasmodium falciparum

Hella C W Fries, Marieke B A C Lamers, Jan Van Deursen, Thivi Ponnudurai, Joseph H E Th Meuwissen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Synthesis of the 25-kDa protein in the early midgut stages of Plasmodium falciparum was studied, using metabolic inhibitors (colchicine and actinomycin D) and pulse-labeling experiments. Experiments with colchicine showed that, immediately after induction of macrogametogenesis, 25-kDa protein synthesis occurs in both fertilized and nonfertilized macrogametes. The amount of 25-kDa protein synthesized increased slowly during time. Experiments with actinomycin D revealed that the slow increase of synthesis may be dependent on de novo messenger RNA synthesis.

Original languageEnglish (US)
Pages (from-to)229-235
Number of pages7
JournalExperimental Parasitology
Volume71
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Zygote
Plasmodium falciparum
Colchicine
Dactinomycin
Proteins
Messenger RNA

Keywords

  • Actinomycin D
  • Colchicine
  • Plasmoduim falciparum
  • Pulse labeling

ASJC Scopus subject areas

  • Immunology
  • Parasitology
  • Infectious Diseases

Cite this

Biosynthesis of the 25-kDa protein in the macrogametes/zygotes of Plasmodium falciparum. / Fries, Hella C W; Lamers, Marieke B A C; Van Deursen, Jan; Ponnudurai, Thivi; Meuwissen, Joseph H E Th.

In: Experimental Parasitology, Vol. 71, No. 2, 1990, p. 229-235.

Research output: Contribution to journalArticle

Fries, Hella C W ; Lamers, Marieke B A C ; Van Deursen, Jan ; Ponnudurai, Thivi ; Meuwissen, Joseph H E Th. / Biosynthesis of the 25-kDa protein in the macrogametes/zygotes of Plasmodium falciparum. In: Experimental Parasitology. 1990 ; Vol. 71, No. 2. pp. 229-235.
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