Biological effects of the plant-derived polyphenol resveratrol in human articular cartilage and chondrosarcoma cells

Hee Jeong Im, Xin Li, Di Chen, Dongyao Yan, Jaesung Kim, Michael B. Ellman, Gary S. Stein, Brian Cole, Ranjan Kc, Gabriella Cs-Szabo, Andre J van Wijnen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The natural phytoestrogen resveratrol (RSV) may have therapeutic potential for arthritic conditions. RSV is chondroprotective for articular cartilage in rabbit models for arthritis, but its biological effects on human articular cartilage and chondrosarcoma cells are unknown. Effects of RSV on human articular cartilage homeostasis were studied by assessing production of matrix-degrading enzymes (MMP-13, ADAMTS4, and ADAMTS5), as well as proteoglycan production and synthesis. The counteractions of RSV against catabolic factors (e.g., FGF-2 or IL-1β) were examined by in vitro and ex vivo using monolayer, three-dimensional alginate beads and cartilage explants cultures, respectively. RSV improves cell viability of articular chondrocytes and effectively antagonizes cartilage-degrading protease production that was initiated by catabolic and/or anti-anabolic cytokines in human articular chondrocytes. RSV significantly also enhances BMP7-promoted proteoglycan synthesis as assessed by 35S-sulfate incorporation. Protein-DNA interaction arrays suggest that RSV inhibits the activation of transcription factors involved in inflammation and cartilage catabolic signaling pathways, including direct downstream regulators of MAPK (e.g., AP-1, PEA3) and NFκB. RSV selectively compromises survival of human chondrosarcoma cells, but not primary articular chondrocytes, revealing cell-specific activity of RSV on non-tumorigenic versus tumor-derived cells. We propose that RSV exerts its chondroprotective functions, in part, by deactivating p53-induced apoptosis in human primary chondrocytes, but not human chondrosarcoma. Our findings suggest that RSV has potential as a unique biologic treatment for both prevention and treatment of cartilage degenerative diseases. J. Cell. Physiol. 227: 3488-3497, 2012. © 2012 Wiley Periodicals, Inc.

Original languageEnglish (US)
Pages (from-to)3488-3497
Number of pages10
JournalJournal of Cellular Physiology
Volume227
Issue number10
DOIs
StatePublished - Oct 2012
Externally publishedYes

Fingerprint

Chondrosarcoma
Cartilage
Articular Cartilage
Polyphenols
Chondrocytes
Joints
Proteoglycans
Arthritis
resveratrol
Cartilage Diseases
Phytoestrogens
Transcription Factor AP-1
Fibroblast Growth Factor 2
Oligonucleotide Array Sequence Analysis
Matrix Metalloproteinases
Interleukin-1
Cell culture
Sulfates
Tumors
Monolayers

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Biological effects of the plant-derived polyphenol resveratrol in human articular cartilage and chondrosarcoma cells. / Im, Hee Jeong; Li, Xin; Chen, Di; Yan, Dongyao; Kim, Jaesung; Ellman, Michael B.; Stein, Gary S.; Cole, Brian; Kc, Ranjan; Cs-Szabo, Gabriella; van Wijnen, Andre J.

In: Journal of Cellular Physiology, Vol. 227, No. 10, 10.2012, p. 3488-3497.

Research output: Contribution to journalArticle

Im, HJ, Li, X, Chen, D, Yan, D, Kim, J, Ellman, MB, Stein, GS, Cole, B, Kc, R, Cs-Szabo, G & van Wijnen, AJ 2012, 'Biological effects of the plant-derived polyphenol resveratrol in human articular cartilage and chondrosarcoma cells', Journal of Cellular Physiology, vol. 227, no. 10, pp. 3488-3497. https://doi.org/10.1002/jcp.24049
Im, Hee Jeong ; Li, Xin ; Chen, Di ; Yan, Dongyao ; Kim, Jaesung ; Ellman, Michael B. ; Stein, Gary S. ; Cole, Brian ; Kc, Ranjan ; Cs-Szabo, Gabriella ; van Wijnen, Andre J. / Biological effects of the plant-derived polyphenol resveratrol in human articular cartilage and chondrosarcoma cells. In: Journal of Cellular Physiology. 2012 ; Vol. 227, No. 10. pp. 3488-3497.
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