Biological Consequences of Overexpression of a Transfected c-erbB-2 Gene in Immortalized Human Bronchial Epithelial Cells

Masayuki Noguchi, Masakazu Murakami, William Bennett, Ruth Lupu, Ferdinand Hui, Curtis C. Harris, Brenda I. Gerwin

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

In order to examine the effects of the overexpression of c-erbB-2 (HER-2, neu) on human bronchial epithelial cells, a human c-erbB-2 expression vector was introduced into the simian virus 40 large T-antigen-immortalized human bronchial epithelial cell line BEAS-2B. Isolation of multiple clonal cell lines after selection revealed a wide range of expression of the gene product gp185erbB-2. While three of six clones tested expressed gp185erbB-2 at levels detectable by immunocytochemistry, only one, B2BE6, induced adenocarcinoma-like tumors in athymic nude mice. Both a nontumorigenic clone, B2BE2, and a tumorigenic clone, B2BE6, expressed comparable amounts of gp185erbB-2, which became phosphory-lated on tyrosine in response to treatment with the c-erbB-2 ligands gp30 and p75. These data suggest that overexpression of c-erbB-2 in human bronchial epithelial cells can contribute to, but is not sufficient for, induction of tumorigenicity in this human model system.

Original languageEnglish (US)
Pages (from-to)2035-2043
Number of pages9
JournalCancer research
Volume53
Issue number9
StatePublished - May 1993

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Noguchi, M., Murakami, M., Bennett, W., Lupu, R., Hui, F., Harris, C. C., & Gerwin, B. I. (1993). Biological Consequences of Overexpression of a Transfected c-erbB-2 Gene in Immortalized Human Bronchial Epithelial Cells. Cancer research, 53(9), 2035-2043.