Biological consequences of overexpression of a transfected c-erbB-2 gene in immortalized human bronchial epithelial cells

Masayuki Noguchi, Masakazu Murakami, William Bennett, Ruth Lupu, Ferdinand Hui, Curtis C. Harris, Brenda I. Gerwin

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

In order to examine the effects of the overexpression of c-erbB-2 (HER-2, neu) on human bronchial epithelial cells, a human c-erbB-2 expression vector was introduced into the simian virus 40 large T-antigen-immortalized human bronchial epithelial cell line BEAS-2B. Isolation of multiple clonal cell lines after selection revealed a wide range of expression of the gene product gp185erbB-2. While three of six clones tested expressed gp185erbB-2 at levels detectable by immunocytochemistry, only one, B2BE6, induced adenocarcinoma-like tumors in athymic nude mice. Both a nontumorigenic clone, B2BE2, and a tumorigenic clone, B2BE6, expressed comparable amounts of gp185erbB-2, which became phosphorylated on tyrosine in response to treatment with the c-erbB-2 ligands gp30 and p75. These data suggest that overexpression of c-erbB-2 in human bronchial epithelial cells can contribute to, but is not sufficient for, induction of tumorigenicity in this human model system.

Original languageEnglish (US)
Pages (from-to)2035-2043
Number of pages9
JournalCancer Research
Volume53
Issue number9
StatePublished - May 1 1993
Externally publishedYes

Fingerprint

erbB-2 Genes
Epithelial Cells
Clone Cells
Nude Mice
Cell Line
Simian virus 40
Viral Tumor Antigens
Tyrosine
Adenocarcinoma
Immunohistochemistry
Ligands
Gene Expression
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Noguchi, M., Murakami, M., Bennett, W., Lupu, R., Hui, F., Harris, C. C., & Gerwin, B. I. (1993). Biological consequences of overexpression of a transfected c-erbB-2 gene in immortalized human bronchial epithelial cells. Cancer Research, 53(9), 2035-2043.

Biological consequences of overexpression of a transfected c-erbB-2 gene in immortalized human bronchial epithelial cells. / Noguchi, Masayuki; Murakami, Masakazu; Bennett, William; Lupu, Ruth; Hui, Ferdinand; Harris, Curtis C.; Gerwin, Brenda I.

In: Cancer Research, Vol. 53, No. 9, 01.05.1993, p. 2035-2043.

Research output: Contribution to journalArticle

Noguchi, M, Murakami, M, Bennett, W, Lupu, R, Hui, F, Harris, CC & Gerwin, BI 1993, 'Biological consequences of overexpression of a transfected c-erbB-2 gene in immortalized human bronchial epithelial cells', Cancer Research, vol. 53, no. 9, pp. 2035-2043.
Noguchi, Masayuki ; Murakami, Masakazu ; Bennett, William ; Lupu, Ruth ; Hui, Ferdinand ; Harris, Curtis C. ; Gerwin, Brenda I. / Biological consequences of overexpression of a transfected c-erbB-2 gene in immortalized human bronchial epithelial cells. In: Cancer Research. 1993 ; Vol. 53, No. 9. pp. 2035-2043.
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