Biological activity of rhBMP-2 released from PLGA microspheres

J. B. Oldham, L. Lu, X. Zhu, B. D. Porter, T. E. Hefferan, D. R. Larson, B. L. Currier, A. G. Mikos, M. J. Yaszemski

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83 Scopus citations

Abstract

Human recombinant bone morphogenetic protein-2 (rhBMP-2) has been proven effective in stimulating the regeneration of bone in both skeletal and extraskeletal locations. Through encapsulation within, and release from, biodegradable poly(DL-lactic-co-glycolic acid) (PLGA) microspheres, a proven vehicle for sustained delivery of various proteins, the local concentrations of rhBMP-2 could be maintained at optimal levels to stimulate bone regeneration and remodeling at the site of healing in diverse clinical settings. Thus the purpose of this work was to investigate the encapsulation of rhBMP-2 in PLGA microspheres and its biologic activity upon release. Using in vitro tests in simulated body fluids, the effect of rhBMP-2 released from PLGA microspheres upon osteoblast cell cultures was found to be statistically similar to the effect produced by positive controls consisting of nonencapsulated aqueous rhBMP-2 in simulated body fluids. This clarifies an important step in skeletal tissue engineering strategies aimed at the use of encapsulated rhBMP-2 to stimulate bone regeneration and remodeling.

Original languageEnglish (US)
Pages (from-to)289-292
Number of pages4
JournalJournal of Biomechanical Engineering
Volume122
Issue number3
DOIs
StatePublished - Jun 1 2000

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Keywords

  • Biodegradable
  • Bone Morphogenetic Protein (BMP)
  • Microsphere
  • Poly(DL- lactic-co-glycolic acid) (PLGA)
  • Tissue Engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physiology (medical)

Cite this

Oldham, J. B., Lu, L., Zhu, X., Porter, B. D., Hefferan, T. E., Larson, D. R., Currier, B. L., Mikos, A. G., & Yaszemski, M. J. (2000). Biological activity of rhBMP-2 released from PLGA microspheres. Journal of Biomechanical Engineering, 122(3), 289-292. https://doi.org/10.1115/1.429662