Biological activity of rhBMP-2 released from PLGA microspheres

J. B. Oldham, Lichun Lu, X. Zhu, B. D. Porter, T. E. Hefferan, D. R. Larson, B. L. Currier, A. G. Mikos, Michael J Yaszemski

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Human recombinant bone morphogenetic protein-2 (rhBMP-2) has been proven effective in stimulating the regeneration of bone in both skeletal and extraskeletal locations. Through encapsulation within, and release from, biodegradable poly(DL-lactic-co-glycolic acid) (PLGA) microspheres, a proven vehicle for sustained delivery of various proteins, the local concentrations of rhBMP-2 could be maintained at optimal levels to stimulate bone regeneration and remodeling at the site of healing in diverse clinical settings. Thus the purpose of this work was to investigate the encapsulation of rhBMP-2 in PLGA microspheres and its biologic activity upon release. Using in vitro tests in simulated body fluids, the effect of rhBMP-2 released from PLGA microspheres upon osteoblast cell cultures was found to be statistically similar to the effect produced by positive controls consisting of nonencapsulated aqueous rhBMP-2 in simulated body fluids. This clarifies an important step in skeletal tissue engineering strategies aimed at the use of encapsulated rhBMP-2 to stimulate bone regeneration and remodeling.

Original languageEnglish (US)
Pages (from-to)289-292
Number of pages4
JournalJournal of Biomechanical Engineering
Volume122
Issue number3
DOIs
StatePublished - Jun 2000

Fingerprint

Bone Regeneration
Bioactivity
Microspheres
Bone
Bone Remodeling
Body Fluids
Acids
Body fluids
Encapsulation
Tissue Engineering
Proteins
Osteoblasts
Cell Culture Techniques
Tissue engineering
Cell culture
Milk
polylactic acid-polyglycolic acid copolymer

Keywords

  • Biodegradable
  • Bone Morphogenetic Protein (BMP)
  • Microsphere
  • Poly(DL- lactic-co-glycolic acid) (PLGA)
  • Tissue Engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biophysics

Cite this

Biological activity of rhBMP-2 released from PLGA microspheres. / Oldham, J. B.; Lu, Lichun; Zhu, X.; Porter, B. D.; Hefferan, T. E.; Larson, D. R.; Currier, B. L.; Mikos, A. G.; Yaszemski, Michael J.

In: Journal of Biomechanical Engineering, Vol. 122, No. 3, 06.2000, p. 289-292.

Research output: Contribution to journalArticle

Oldham, JB, Lu, L, Zhu, X, Porter, BD, Hefferan, TE, Larson, DR, Currier, BL, Mikos, AG & Yaszemski, MJ 2000, 'Biological activity of rhBMP-2 released from PLGA microspheres', Journal of Biomechanical Engineering, vol. 122, no. 3, pp. 289-292. https://doi.org/10.1115/1.429662
Oldham, J. B. ; Lu, Lichun ; Zhu, X. ; Porter, B. D. ; Hefferan, T. E. ; Larson, D. R. ; Currier, B. L. ; Mikos, A. G. ; Yaszemski, Michael J. / Biological activity of rhBMP-2 released from PLGA microspheres. In: Journal of Biomechanical Engineering. 2000 ; Vol. 122, No. 3. pp. 289-292.
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