Biological activity of a new neuronal growth factor from injured peripheral nerve

Anthony J. Windebank, Marceil D. Blexrud

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

In response to transection injury, the distal nerve segment produces a soluble neurite promoting factor (SN). In this study, the ability to support neuronal survival and differentiation have been studied. Embryonic rat dorsal root ganglion (DRG) neurons were plated out on collagen substrates and incubated in medium containing either SN or nerve growth factor (NGF). The number of surviving neurons was counted after I, 2, 4, 7, and 15 days in vitro. After fixation and staining, the diameter of the surviving neurons was measured. During the period of observation, 60.8 ± 5.8% of plated neurons survived in the presence of NGF and 90.5 ± 12.9% survived with SN (P < 0.05). The mean of median neuronal cell diameter was 28 ± 2.7 μm with NGF and 34.2 ± 3.7 μm with SN, (P < 0.01). This increased diameter was due to enhanced survival of 30-50 μm diameter neurons. In parallel experiments, the degree of myelination of DRG neurons by Schwann cells was assessed morphometrically. In the presence of SN there was an 86% increase in myelination compared with NGF which indicates that not only is the survival of neurons increased but they are able to become fully differentiated in the presence of SN.

Original languageEnglish (US)
Pages (from-to)243-251
Number of pages9
JournalDevelopmental Brain Research
Volume49
Issue number2
DOIs
StatePublished - Oct 1 1989

Keywords

  • Axon-Schwann cell interaction
  • Dorsal root ganglion neuron
  • Myelination
  • Neuronal growth factor

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

Fingerprint Dive into the research topics of 'Biological activity of a new neuronal growth factor from injured peripheral nerve'. Together they form a unique fingerprint.

  • Cite this