Biologic and clinical significance of basic fibroblast growth factor immunostaining in breast carcinoma

D. W. Visscher, F. DeMattia, S. Ottosen, F. H. Sarkar, J. D. Crissman

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Acetone-fixed cryostat sections of 79 breast carcinomas were immunostained with antibodies to basic fibroblast growth factor (bFGF) and urokinase-type plasminogen activator (uPA). Staining intensity was then compared with microvessel density assessed by manually counting vascular spaces highlighted by immunostaining vascular basal lamina (Type IV) collagen. Extensive (2+) bFGF immunoreactivity was present in neoplastic cells of 30 tumors (38%) and in host-derived stromal cells of 29 cases (37%). Disease recurrence correlated with bFGF staining: 0 to 1+ stromal staining, 30% recurred versus 2+ stromal staining, 73% recurred (P = 0.001) (54-mo median follow-up). Neither stromal nor epithelial bFGF staining correlated significantly with microvessel count; however, there was a statistically significant association between stromal cell bFGF staining and uPA staining of peritumor host cells: absent bFGF -0% 2+ uPA versus weak bFGF -9% 2+ uPA versus 2+ bFGF -29% 2+ uPA (P = 0.01). We conclude that elevated expression of bFGF in breast carcinomas is associated with aggressive clinical behavior. Its biologic significance, however, appears more closely related to extracellular matrix remodeling than to induction of prominent neovascularization per se.

Original languageEnglish (US)
Pages (from-to)665-670
Number of pages6
JournalModern Pathology
Volume8
Issue number6
StatePublished - Jan 1 1995

Keywords

  • Angiogenesis
  • Basic fibroblast growth factor
  • Breast carcinoma
  • Urokinase plasminogen activator

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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