Biofilm development in laboratory methanogenic fluidized bed reactors

L. G M Gorris, Jan Van Deursen, C. van der Drift, G. D. Vogels

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Biofilm development on sand with different heterogeneous inocula was studied in laboratory‐scale methanogenic fluidized bed reactors. Both the course of biofilm formation during reactor start‐up and the bacterial composition of newly developed biofilms at steady‐state were found to be similar irrespective of the type of inoculum applied. Biofilm formation proceeded according to a fixed pattern that could be subdivided in three consecutive phases, designated as the lag phase, biofilm production phase, and steady‐state phase. Methanogenic activity and biomass content of the fluidized bed granules were found to be accurate parameters of the course of biofilm formation. More indirect parameters monitored did not give unambiguous results in all instances. The composition of the newly developed biomass as assessed on the basis of potential methanogenic activities on different substrates and of the concentration of specific methanogenic cofactors was consistent with electron microscopic observations.

Original languageEnglish (US)
Pages (from-to)687-693
Number of pages7
JournalBiotechnology and Bioengineering
Volume33
Issue number6
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Biofilms
Fluidized beds
Biomass
Reactor startup
Chemical analysis
Sand
Electrons
Substrates

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Biofilm development in laboratory methanogenic fluidized bed reactors. / Gorris, L. G M; Van Deursen, Jan; van der Drift, C.; Vogels, G. D.

In: Biotechnology and Bioengineering, Vol. 33, No. 6, 1989, p. 687-693.

Research output: Contribution to journalArticle

Gorris, L. G M ; Van Deursen, Jan ; van der Drift, C. ; Vogels, G. D. / Biofilm development in laboratory methanogenic fluidized bed reactors. In: Biotechnology and Bioengineering. 1989 ; Vol. 33, No. 6. pp. 687-693.
@article{4f8f8f9fa6414da1b8ce16b8be7850e8,
title = "Biofilm development in laboratory methanogenic fluidized bed reactors",
abstract = "Biofilm development on sand with different heterogeneous inocula was studied in laboratory‐scale methanogenic fluidized bed reactors. Both the course of biofilm formation during reactor start‐up and the bacterial composition of newly developed biofilms at steady‐state were found to be similar irrespective of the type of inoculum applied. Biofilm formation proceeded according to a fixed pattern that could be subdivided in three consecutive phases, designated as the lag phase, biofilm production phase, and steady‐state phase. Methanogenic activity and biomass content of the fluidized bed granules were found to be accurate parameters of the course of biofilm formation. More indirect parameters monitored did not give unambiguous results in all instances. The composition of the newly developed biomass as assessed on the basis of potential methanogenic activities on different substrates and of the concentration of specific methanogenic cofactors was consistent with electron microscopic observations.",
author = "Gorris, {L. G M} and {Van Deursen}, Jan and {van der Drift}, C. and Vogels, {G. D.}",
year = "1989",
doi = "10.1002/bit.260330605",
language = "English (US)",
volume = "33",
pages = "687--693",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "6",

}

TY - JOUR

T1 - Biofilm development in laboratory methanogenic fluidized bed reactors

AU - Gorris, L. G M

AU - Van Deursen, Jan

AU - van der Drift, C.

AU - Vogels, G. D.

PY - 1989

Y1 - 1989

N2 - Biofilm development on sand with different heterogeneous inocula was studied in laboratory‐scale methanogenic fluidized bed reactors. Both the course of biofilm formation during reactor start‐up and the bacterial composition of newly developed biofilms at steady‐state were found to be similar irrespective of the type of inoculum applied. Biofilm formation proceeded according to a fixed pattern that could be subdivided in three consecutive phases, designated as the lag phase, biofilm production phase, and steady‐state phase. Methanogenic activity and biomass content of the fluidized bed granules were found to be accurate parameters of the course of biofilm formation. More indirect parameters monitored did not give unambiguous results in all instances. The composition of the newly developed biomass as assessed on the basis of potential methanogenic activities on different substrates and of the concentration of specific methanogenic cofactors was consistent with electron microscopic observations.

AB - Biofilm development on sand with different heterogeneous inocula was studied in laboratory‐scale methanogenic fluidized bed reactors. Both the course of biofilm formation during reactor start‐up and the bacterial composition of newly developed biofilms at steady‐state were found to be similar irrespective of the type of inoculum applied. Biofilm formation proceeded according to a fixed pattern that could be subdivided in three consecutive phases, designated as the lag phase, biofilm production phase, and steady‐state phase. Methanogenic activity and biomass content of the fluidized bed granules were found to be accurate parameters of the course of biofilm formation. More indirect parameters monitored did not give unambiguous results in all instances. The composition of the newly developed biomass as assessed on the basis of potential methanogenic activities on different substrates and of the concentration of specific methanogenic cofactors was consistent with electron microscopic observations.

UR - http://www.scopus.com/inward/record.url?scp=84996081055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84996081055&partnerID=8YFLogxK

U2 - 10.1002/bit.260330605

DO - 10.1002/bit.260330605

M3 - Article

AN - SCOPUS:84996081055

VL - 33

SP - 687

EP - 693

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 6

ER -