Abstract
The biodistribution and resulting pattern of transgene expression were determined following intravesical administration of an adenoviral vector carrying the luciferase reporter gene (AdLuc). Female BALB/c mice were subjected to intravesical instillation of 1 × 109 or 5 × 109 plaque-forming units of AdLuc. After sacrifice, transgene expression was detected in tissues using luciferase assays; vector DNA was detected by vector-specific polymerase chain reaction. These experiments showed very little vector dissemination outside of the bladder by this route of administration. High-level expression of the vector transgene in the bladder was found to diminish by severalfold after 3 days. In a supporting study, vector dissemination and resulting transgene expression were determined following tail vein injection of 5 × 109 plaque-forming units of AdLuc. Vector was distributed to and expressed in every organ analyzed, with the highest concentration and level of expression observed in the liver.
Original language | English (US) |
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Pages (from-to) | 367-372 |
Number of pages | 6 |
Journal | Cancer Gene Therapy |
Volume | 6 |
Issue number | 4 |
DOIs | |
State | Published - 1999 |
Keywords
- AdLuc
- Biodistribution
- Intravesical administration
- Luciferase activity
- Polymerase chain reaction
- Tail vein administration
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Cancer Research