Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse

Mark Wood, Paul Perrotte, Eric Onishi, Mary E. Harper, Colin Dinney, Lance Pagliaro, Deborah R. Wilson

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

The biodistribution and resulting pattern of transgene expression were determined following intravesical administration of an adenoviral vector carrying the luciferase reporter gene (AdLuc). Female BALB/c mice were subjected to intravesical instillation of 1 × 109 or 5 × 109 plaque-forming units of AdLuc. After sacrifice, transgene expression was detected in tissues using luciferase assays; vector DNA was detected by vector-specific polymerase chain reaction. These experiments showed very little vector dissemination outside of the bladder by this route of administration. High-level expression of the vector transgene in the bladder was found to diminish by severalfold after 3 days. In a supporting study, vector dissemination and resulting transgene expression were determined following tail vein injection of 5 × 109 plaque-forming units of AdLuc. Vector was distributed to and expressed in every organ analyzed, with the highest concentration and level of expression observed in the liver.

Original languageEnglish (US)
Pages (from-to)367-372
Number of pages6
JournalCancer Gene Therapy
Volume6
Issue number4
DOIs
StatePublished - 1999

Keywords

  • AdLuc
  • Biodistribution
  • Intravesical administration
  • Luciferase activity
  • Polymerase chain reaction
  • Tail vein administration

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cancer Research

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