Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse

Mark Wood, Paul Perrotte, Eric Onishi, Mary E. Harper, Colin Dinney, Lance Pagliaro, Deborah R. Wilson

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The biodistribution and resulting pattern of transgene expression were determined following intravesical administration of an adenoviral vector carrying the luciferase reporter gene (AdLuc). Female BALB/c mice were subjected to intravesical instillation of 1 × 109 or 5 × 109 plaque-forming units of AdLuc. After sacrifice, transgene expression was detected in tissues using luciferase assays; vector DNA was detected by vector-specific polymerase chain reaction. These experiments showed very little vector dissemination outside of the bladder by this route of administration. High-level expression of the vector transgene in the bladder was found to diminish by severalfold after 3 days. In a supporting study, vector dissemination and resulting transgene expression were determined following tail vein injection of 5 × 109 plaque-forming units of AdLuc. Vector was distributed to and expressed in every organ analyzed, with the highest concentration and level of expression observed in the liver.

Original languageEnglish (US)
Pages (from-to)367-372
Number of pages6
JournalCancer Gene Therapy
Volume6
Issue number4
StatePublished - 1999
Externally publishedYes

Fingerprint

Intravesical Administration
Luciferases
Transgenes
Reporter Genes
Intravenous Administration
Urinary Bladder
Tail
Veins
Polymerase Chain Reaction
Injections
Liver
DNA

Keywords

  • AdLuc
  • Biodistribution
  • Intravesical administration
  • Luciferase activity
  • Polymerase chain reaction
  • Tail vein administration

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Wood, M., Perrotte, P., Onishi, E., Harper, M. E., Dinney, C., Pagliaro, L., & Wilson, D. R. (1999). Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse. Cancer Gene Therapy, 6(4), 367-372.

Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse. / Wood, Mark; Perrotte, Paul; Onishi, Eric; Harper, Mary E.; Dinney, Colin; Pagliaro, Lance; Wilson, Deborah R.

In: Cancer Gene Therapy, Vol. 6, No. 4, 1999, p. 367-372.

Research output: Contribution to journalArticle

Wood, M, Perrotte, P, Onishi, E, Harper, ME, Dinney, C, Pagliaro, L & Wilson, DR 1999, 'Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse', Cancer Gene Therapy, vol. 6, no. 4, pp. 367-372.
Wood, Mark ; Perrotte, Paul ; Onishi, Eric ; Harper, Mary E. ; Dinney, Colin ; Pagliaro, Lance ; Wilson, Deborah R. / Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse. In: Cancer Gene Therapy. 1999 ; Vol. 6, No. 4. pp. 367-372.
@article{f445f7aa8161400191490d86f258c28e,
title = "Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse",
abstract = "The biodistribution and resulting pattern of transgene expression were determined following intravesical administration of an adenoviral vector carrying the luciferase reporter gene (AdLuc). Female BALB/c mice were subjected to intravesical instillation of 1 × 109 or 5 × 109 plaque-forming units of AdLuc. After sacrifice, transgene expression was detected in tissues using luciferase assays; vector DNA was detected by vector-specific polymerase chain reaction. These experiments showed very little vector dissemination outside of the bladder by this route of administration. High-level expression of the vector transgene in the bladder was found to diminish by severalfold after 3 days. In a supporting study, vector dissemination and resulting transgene expression were determined following tail vein injection of 5 × 109 plaque-forming units of AdLuc. Vector was distributed to and expressed in every organ analyzed, with the highest concentration and level of expression observed in the liver.",
keywords = "AdLuc, Biodistribution, Intravesical administration, Luciferase activity, Polymerase chain reaction, Tail vein administration",
author = "Mark Wood and Paul Perrotte and Eric Onishi and Harper, {Mary E.} and Colin Dinney and Lance Pagliaro and Wilson, {Deborah R.}",
year = "1999",
language = "English (US)",
volume = "6",
pages = "367--372",
journal = "Cancer Gene Therapy",
issn = "0929-1903",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse

AU - Wood, Mark

AU - Perrotte, Paul

AU - Onishi, Eric

AU - Harper, Mary E.

AU - Dinney, Colin

AU - Pagliaro, Lance

AU - Wilson, Deborah R.

PY - 1999

Y1 - 1999

N2 - The biodistribution and resulting pattern of transgene expression were determined following intravesical administration of an adenoviral vector carrying the luciferase reporter gene (AdLuc). Female BALB/c mice were subjected to intravesical instillation of 1 × 109 or 5 × 109 plaque-forming units of AdLuc. After sacrifice, transgene expression was detected in tissues using luciferase assays; vector DNA was detected by vector-specific polymerase chain reaction. These experiments showed very little vector dissemination outside of the bladder by this route of administration. High-level expression of the vector transgene in the bladder was found to diminish by severalfold after 3 days. In a supporting study, vector dissemination and resulting transgene expression were determined following tail vein injection of 5 × 109 plaque-forming units of AdLuc. Vector was distributed to and expressed in every organ analyzed, with the highest concentration and level of expression observed in the liver.

AB - The biodistribution and resulting pattern of transgene expression were determined following intravesical administration of an adenoviral vector carrying the luciferase reporter gene (AdLuc). Female BALB/c mice were subjected to intravesical instillation of 1 × 109 or 5 × 109 plaque-forming units of AdLuc. After sacrifice, transgene expression was detected in tissues using luciferase assays; vector DNA was detected by vector-specific polymerase chain reaction. These experiments showed very little vector dissemination outside of the bladder by this route of administration. High-level expression of the vector transgene in the bladder was found to diminish by severalfold after 3 days. In a supporting study, vector dissemination and resulting transgene expression were determined following tail vein injection of 5 × 109 plaque-forming units of AdLuc. Vector was distributed to and expressed in every organ analyzed, with the highest concentration and level of expression observed in the liver.

KW - AdLuc

KW - Biodistribution

KW - Intravesical administration

KW - Luciferase activity

KW - Polymerase chain reaction

KW - Tail vein administration

UR - http://www.scopus.com/inward/record.url?scp=0033161662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033161662&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 367

EP - 372

JO - Cancer Gene Therapy

JF - Cancer Gene Therapy

SN - 0929-1903

IS - 4

ER -