Biochemical evidence for trans complementation of structural genes in the expression of I-E antigens in F1 hybrids

M. L. Plunkett, C. S. David, J. H. Freed

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Analysis of the tryptic peptides obtained from the Ia. 7-positive, 'I-E' antigens produced by 4 F1 combinations derived from stain A TFR5 has provided biochemical confirmation that I-A/I-E gene complementation can occur in the trans configuration. The data obtained support the concept that structural genes, as opposed to regulatory genes, are the essential elements involved in this complementation and support the assignment of the I-E subregion of strain A.TFR5 as I-E(k). The data support the following assignments of the genes encoding the E(β) chains: B10.A(4R) as E(β)(k), B10.A(5R) as E(β)(b), and B10.HTT as E(β)(S). Finally, data are presented demonstrating that the E(β) chain derived from the recombinant D2.GD strain partially differs from the E(β)(d) chain derived from a conventional H-2(d) haplotype strain, B10.D2.

Original languageEnglish (US)
Pages (from-to)1679-1685
Number of pages7
JournalJournal of Immunology
Volume127
Issue number4
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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