Biochemical documentation of allelic variation of the I-E antigens of the d, k, p, r, and u haplotypes

John M. Kupinski; Pamela A. Gamble; Chella S. David; John H. Freed

doi:10.1007/BF00372099

Biochemical documentation of allelic variation of the I-E antigens of the d, k, p, r, and u haplotypes

John M. Kupinski, Pamela A. Gamble, Chella S. David, John H. Freed

Immunology

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6 Scopus citations

Abstract

The extent of allelic variation of the E_α and E_β polypeptide chains of the I-E antigens from the H-2> ^d, H-2^k, H-2^p, H-2^r, and H-2^u haplotypes is described. E_α and E_β chains were individually labeled with arginine or lysine and compared by tryptic peptide analysis. The results indicate minimum variability among the E_α polypeptides encoded by the d, k, p, and r haplotypes. However, the E_α^uchain differed significantly from the other allelic E_α gene products. On the other hand, the E_β alleles demonstrated substantial variability with the E_β^dbeing notably less similar to the other alleles than they are to each other. These findings are consistent with a number of observations regarding the serology and functions of the I-E antigens.

Original language	English (US)
Pages (from-to)	393-405
Number of pages	13
Journal	Immunogenetics
Volume	16
Issue number	5
DOIs	https://doi.org/10.1007/BF00372099
State	Published - Nov 1982

ASJC Scopus subject areas

Immunology
Genetics

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title = "Biochemical documentation of allelic variation of the I-E antigens of the d, k, p, r, and u haplotypes",

abstract = "The extent of allelic variation of the Eα and Eβ polypeptide chains of the I-E antigens from the H-2> d, H-2k, H-2p, H-2r, and H-2u haplotypes is described. Eα and Eβ chains were individually labeled with arginine or lysine and compared by tryptic peptide analysis. The results indicate minimum variability among the Eα polypeptides encoded by the d, k, p, and r haplotypes. However, the Eαuchain differed significantly from the other allelic Eα gene products. On the other hand, the Eβ alleles demonstrated substantial variability with the Eβdbeing notably less similar to the other alleles than they are to each other. These findings are consistent with a number of observations regarding the serology and functions of the I-E antigens.",

author = "Kupinski, {John M.} and Gamble, {Pamela A.} and David, {Chella S.} and Freed, {John H.}",

year = "1982",

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pages = "393--405",

journal = "Immunogenetics",

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TY - JOUR

T1 - Biochemical documentation of allelic variation of the I-E antigens of the d, k, p, r, and u haplotypes

AU - Kupinski, John M.

AU - Gamble, Pamela A.

AU - David, Chella S.

AU - Freed, John H.

PY - 1982/11

Y1 - 1982/11

N2 - The extent of allelic variation of the Eα and Eβ polypeptide chains of the I-E antigens from the H-2> d, H-2k, H-2p, H-2r, and H-2u haplotypes is described. Eα and Eβ chains were individually labeled with arginine or lysine and compared by tryptic peptide analysis. The results indicate minimum variability among the Eα polypeptides encoded by the d, k, p, and r haplotypes. However, the Eαuchain differed significantly from the other allelic Eα gene products. On the other hand, the Eβ alleles demonstrated substantial variability with the Eβdbeing notably less similar to the other alleles than they are to each other. These findings are consistent with a number of observations regarding the serology and functions of the I-E antigens.

AB - The extent of allelic variation of the Eα and Eβ polypeptide chains of the I-E antigens from the H-2> d, H-2k, H-2p, H-2r, and H-2u haplotypes is described. Eα and Eβ chains were individually labeled with arginine or lysine and compared by tryptic peptide analysis. The results indicate minimum variability among the Eα polypeptides encoded by the d, k, p, and r haplotypes. However, the Eαuchain differed significantly from the other allelic Eα gene products. On the other hand, the Eβ alleles demonstrated substantial variability with the Eβdbeing notably less similar to the other alleles than they are to each other. These findings are consistent with a number of observations regarding the serology and functions of the I-E antigens.

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Biochemical documentation of allelic variation of the I-E antigens of the d, k, p, r, and u haplotypes

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