TY - JOUR
T1 - Biochemical differentiation of mechanically dissociated mammalian brain in aggregating cell culture
AU - Honegger, Paul
AU - Richelson, Elliott
N1 - Funding Information:
These experiments were carried out at the Department of Pharmacology and Experimental Therapeutics, The Johns Hopkins Univelsity School of Medicine, Baltimore, Md., where P.H. was recipient of a fellowship from the Mellon Foundation. We wish to thank Ms. Roxanna Thompson for her excellent assistance in the dissection of the embryos.
Funding Information:
This work was supported in part by United States Public Health Service Grant No. MH27692.
PY - 1976/6/11
Y1 - 1976/6/11
N2 - Mouse and rat brain cells were dissociated by a simple mechanical sieving technique and studied in culture for the formation of aggregates and the activities of choline acetyltransferase, acetylcholinesterase, glutamic acid decarboxylase, tyrosine 3-monooxygenase, aromatic l-amino acid decarboxylase, catechol methyltransferase, and monoamine oxidase. Cells from fetal and neonatal tissue formed aggregates but not cells from tissue older than two days after birth. The pattern of development of enzyme activities in these aggregates varied with the age of the starting tissue. The highest levels of specific activity for the neuron-specific enzymes were found after 3-4 weeks in culture for aggregates of cells derived from relatively undeveloped brains.
AB - Mouse and rat brain cells were dissociated by a simple mechanical sieving technique and studied in culture for the formation of aggregates and the activities of choline acetyltransferase, acetylcholinesterase, glutamic acid decarboxylase, tyrosine 3-monooxygenase, aromatic l-amino acid decarboxylase, catechol methyltransferase, and monoamine oxidase. Cells from fetal and neonatal tissue formed aggregates but not cells from tissue older than two days after birth. The pattern of development of enzyme activities in these aggregates varied with the age of the starting tissue. The highest levels of specific activity for the neuron-specific enzymes were found after 3-4 weeks in culture for aggregates of cells derived from relatively undeveloped brains.
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U2 - 10.1016/0006-8993(76)90534-5
DO - 10.1016/0006-8993(76)90534-5
M3 - Article
C2 - 6121
AN - SCOPUS:0017070315
SN - 0006-8993
VL - 109
SP - 335
EP - 354
JO - Brain Research
JF - Brain Research
IS - 2
ER -