Biochemical differentiation of mechanically dissociated mammalian brain in aggregating cell culture

Paul Honegger, Elliott Richelson

Research output: Contribution to journalArticle

159 Scopus citations

Abstract

Mouse and rat brain cells were dissociated by a simple mechanical sieving technique and studied in culture for the formation of aggregates and the activities of choline acetyltransferase, acetylcholinesterase, glutamic acid decarboxylase, tyrosine 3-monooxygenase, aromatic l-amino acid decarboxylase, catechol methyltransferase, and monoamine oxidase. Cells from fetal and neonatal tissue formed aggregates but not cells from tissue older than two days after birth. The pattern of development of enzyme activities in these aggregates varied with the age of the starting tissue. The highest levels of specific activity for the neuron-specific enzymes were found after 3-4 weeks in culture for aggregates of cells derived from relatively undeveloped brains.

Original languageEnglish (US)
Pages (from-to)335-354
Number of pages20
JournalBrain Research
Volume109
Issue number2
DOIs
StatePublished - Jun 11 1976

    Fingerprint

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this