Biochemical and immunological characterization of neurofilaments in experimental neurofibrillary degeneration induced by aluminum

Dennis J. Selkoe, Ronald K H Liem, Shu Hui Yen, Michael L. Shelanski

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

In order to identify the protein composition of 10 nm neuronal filaments, we prepared enriched fractions of rabbit spinal neurons undergoing experimental neurofilamentous degeneration induced by aluminum. Electron microscopy of the isolated perikarya showed well-preserved, large perinuclear masses of neurofilaments, which were not found in similarly isolated control perikarya. Comparison of these glial-free fractions by SDS-polyacrylamide gel electrophoresis revealed several-fold augmentation in the filament-enriched neurons of proteins migrating at 68,000 and 160,000 daltons, with an additional component at 200,000 daltons. Otherwise, the protein patterns were identical; no band was found at 51,000 daltons, the molecular weight assigned to the major proteins both of glial filaments and of a previously reported bovine brain filament preparation. An antiserum raised against the 160,000 dalton component of a modified bovine brain filament fraction produced specific and intense fluorescent staining of the aluminum-induced neurofilament bundles. Antibodies to the 51,000 dalton protein of brain filaments and to tubulin failed to stain the induced filaments. The results strongly support the hypothesis that both normal and aluminum-induced neuronal filaments are composed of 68,000, 160,000 and 200,000 dalton polypeptides and do not contain significant amounts of the 51,000 dalton filament protein. The likelihood of biochemical heterogeneity among organelles with similar morphology, namely the glial and neuronal filaments, is raised.

Original languageEnglish (US)
Pages (from-to)235-252
Number of pages18
JournalBrain Research
Volume163
Issue number2
DOIs
StatePublished - Mar 16 1979
Externally publishedYes

Fingerprint

Intermediate Filaments
Aluminum
Neuroglia
Proteins
Brain
Neurons
Tubulin
Organelles
Immune Sera
Polyacrylamide Gel Electrophoresis
Electron Microscopy
Coloring Agents
Molecular Weight
Staining and Labeling
Rabbits
Peptides
Antibodies

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Biochemical and immunological characterization of neurofilaments in experimental neurofibrillary degeneration induced by aluminum. / Selkoe, Dennis J.; Liem, Ronald K H; Yen, Shu Hui; Shelanski, Michael L.

In: Brain Research, Vol. 163, No. 2, 16.03.1979, p. 235-252.

Research output: Contribution to journalArticle

Selkoe, Dennis J. ; Liem, Ronald K H ; Yen, Shu Hui ; Shelanski, Michael L. / Biochemical and immunological characterization of neurofilaments in experimental neurofibrillary degeneration induced by aluminum. In: Brain Research. 1979 ; Vol. 163, No. 2. pp. 235-252.
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