Biochemical actions of follicle stimulating hormone in the Sertoli cell of the rat testis

J. L. Fakunding, D. J. Tindall, J. R. Dedman

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The sequence of biochemical events associated with the action of follicle stimulating hormone (FSH) in the testis has been investigated using a Sertoli cell enriched testis model system. The Sertoli cell enriched testis, created by irradiation of male rats in utero, is devoid of germinal elements but contains a normal complement of supportive Sertoli cells. Comparison of the Sertoli cell enriched testis with normal testis, demonstrates that the two types of testes contain equal numbers of FSH specific receptors, judged by the binding of labeled hormone. In addition, FSH over a concentration range from 6 x 10-11 to 6 x 10-9M will stimulate the production of adenosine 3',5' monophosphate (cAMP) in the Sertoli cell enriched testis in a manner indistinguishable from that of the normal testis. Incubation of Sertoli cell enriched testis also results in the activation of soluble cAMP dependent protein kinase. This response to FSH is dependent upon the age of the animal and disappears at about 32 days of age. While sensitivity to the hormone can still be detected in mature Sertoli cell enriched animals by the addition of the phosphodiesterase inhibitor 1 methyl 3 isobutylxanthine, no detectable increase in phosphodiesterase activity is apparent after 30 days of age. Injections of FSH into Sertoli cell enriched animals results in an increase in total testicular protein synthesis as well as in the production of the Sertoli cell specific protein, androgen binding protein within 30 min. Furthermore, while hypophysectomy of Sertoli cell enriched animals results in a decline of the testicular concentration of androgen binding protein, the injection of FSH will stimulate and maintain the levels of androgen binding protein in such animals. These results demonstrate that the Sertoli cell enriched testis is capable of carrying out the sequence of biochemical events previously described for FSH in the normal testis and therefore, suggest that the Sertoli cell is the primary target for FSH action.

Original languageEnglish (US)
Pages (from-to)392-402
Number of pages11
JournalEndocrinology
Volume98
Issue number2
StatePublished - 1976
Externally publishedYes

Fingerprint

Sertoli Cells
Follicle Stimulating Hormone
Testis
Androgen-Binding Protein
Hormones
FSH Receptors
1-Methyl-3-isobutylxanthine
Hypophysectomy
Injections
Phosphodiesterase Inhibitors
Phosphoric Diester Hydrolases
Cyclic AMP-Dependent Protein Kinases
Adenosine
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Fakunding, J. L., Tindall, D. J., & Dedman, J. R. (1976). Biochemical actions of follicle stimulating hormone in the Sertoli cell of the rat testis. Endocrinology, 98(2), 392-402.

Biochemical actions of follicle stimulating hormone in the Sertoli cell of the rat testis. / Fakunding, J. L.; Tindall, D. J.; Dedman, J. R.

In: Endocrinology, Vol. 98, No. 2, 1976, p. 392-402.

Research output: Contribution to journalArticle

Fakunding, JL, Tindall, DJ & Dedman, JR 1976, 'Biochemical actions of follicle stimulating hormone in the Sertoli cell of the rat testis', Endocrinology, vol. 98, no. 2, pp. 392-402.
Fakunding, J. L. ; Tindall, D. J. ; Dedman, J. R. / Biochemical actions of follicle stimulating hormone in the Sertoli cell of the rat testis. In: Endocrinology. 1976 ; Vol. 98, No. 2. pp. 392-402.
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