Bioassay of EDRF from internal mammary arteries

Implications for early and late bypass graft patency

Paul J. Pearson, Paulo R B Evora, Hartzell V Schaff

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

To study the basal, luminal release of endothelium-derived relaxing factor, 35-mm segments of canine internal mammary artery (IMA) were cannulated and perfused at 5 mL/min in vitro with physiological salt solution. Vasoactive properties of the effluent were bioassayed on coronary artery smooth muscle. Effluent from IMAs produced significant vasodilation of the bioassay ring compared with effluent from a prosthetic conduit (n = 24; p < 0.05). The vasodilation by the effluent could be eliminated by mechanically removing the intima of the IMA, or by treating the IMA segments with NG-monomethyl-l-arginine or NG-nitro-l-arginine, two competitive inhibitors of nitric oxide synthesis from l-arginine; vasodilation was not influenced by treatment with indomethacin. In 83% of the superfusion experiments, effluent from the left IMA induced greater relaxation of the bioassay ring than did effluent from the right IMA. In addition, the average vasodilation induced by left IMA effluent was 28% ± 2.3% versus 17.4% ± 3.1% for the right (n = 24; p < 0.05). However, in organ chamber experiments, right and left IMAs exhibited comparable endothelium-dependent vasodilation to acetylcholine (n = 6). Because endothelium-derived relaxing factor induces vasodilation and also inhibits platelet adhesion, platelet aggregation, and atherogenesis, luminal release of endothelium-derived relaxing factor by the IMA could contribute to superior results when the artery is used in bypass grafting.

Original languageEnglish (US)
Pages (from-to)1078-1084
Number of pages7
JournalThe Annals of Thoracic Surgery
Volume54
Issue number6
DOIs
StatePublished - 1992

Fingerprint

Mammary Arteries
Biological Assay
Vasodilation
Transplants
Endothelium-Dependent Relaxing Factors
Arginine
Platelet Aggregation
Indomethacin
Acetylcholine
Endothelium
Smooth Muscle
Canidae
Atherosclerosis
Coronary Vessels
Nitric Oxide
Blood Platelets
Arteries
Salts

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Bioassay of EDRF from internal mammary arteries : Implications for early and late bypass graft patency. / Pearson, Paul J.; Evora, Paulo R B; Schaff, Hartzell V.

In: The Annals of Thoracic Surgery, Vol. 54, No. 6, 1992, p. 1078-1084.

Research output: Contribution to journalArticle

@article{d3b01ced452e416ea30c1d1b5caeeb38,
title = "Bioassay of EDRF from internal mammary arteries: Implications for early and late bypass graft patency",
abstract = "To study the basal, luminal release of endothelium-derived relaxing factor, 35-mm segments of canine internal mammary artery (IMA) were cannulated and perfused at 5 mL/min in vitro with physiological salt solution. Vasoactive properties of the effluent were bioassayed on coronary artery smooth muscle. Effluent from IMAs produced significant vasodilation of the bioassay ring compared with effluent from a prosthetic conduit (n = 24; p < 0.05). The vasodilation by the effluent could be eliminated by mechanically removing the intima of the IMA, or by treating the IMA segments with NG-monomethyl-l-arginine or NG-nitro-l-arginine, two competitive inhibitors of nitric oxide synthesis from l-arginine; vasodilation was not influenced by treatment with indomethacin. In 83{\%} of the superfusion experiments, effluent from the left IMA induced greater relaxation of the bioassay ring than did effluent from the right IMA. In addition, the average vasodilation induced by left IMA effluent was 28{\%} ± 2.3{\%} versus 17.4{\%} ± 3.1{\%} for the right (n = 24; p < 0.05). However, in organ chamber experiments, right and left IMAs exhibited comparable endothelium-dependent vasodilation to acetylcholine (n = 6). Because endothelium-derived relaxing factor induces vasodilation and also inhibits platelet adhesion, platelet aggregation, and atherogenesis, luminal release of endothelium-derived relaxing factor by the IMA could contribute to superior results when the artery is used in bypass grafting.",
author = "Pearson, {Paul J.} and Evora, {Paulo R B} and Schaff, {Hartzell V}",
year = "1992",
doi = "10.1016/0003-4975(92)90073-D",
language = "English (US)",
volume = "54",
pages = "1078--1084",
journal = "Annals of Thoracic Surgery",
issn = "0003-4975",
publisher = "Elsevier USA",
number = "6",

}

TY - JOUR

T1 - Bioassay of EDRF from internal mammary arteries

T2 - Implications for early and late bypass graft patency

AU - Pearson, Paul J.

AU - Evora, Paulo R B

AU - Schaff, Hartzell V

PY - 1992

Y1 - 1992

N2 - To study the basal, luminal release of endothelium-derived relaxing factor, 35-mm segments of canine internal mammary artery (IMA) were cannulated and perfused at 5 mL/min in vitro with physiological salt solution. Vasoactive properties of the effluent were bioassayed on coronary artery smooth muscle. Effluent from IMAs produced significant vasodilation of the bioassay ring compared with effluent from a prosthetic conduit (n = 24; p < 0.05). The vasodilation by the effluent could be eliminated by mechanically removing the intima of the IMA, or by treating the IMA segments with NG-monomethyl-l-arginine or NG-nitro-l-arginine, two competitive inhibitors of nitric oxide synthesis from l-arginine; vasodilation was not influenced by treatment with indomethacin. In 83% of the superfusion experiments, effluent from the left IMA induced greater relaxation of the bioassay ring than did effluent from the right IMA. In addition, the average vasodilation induced by left IMA effluent was 28% ± 2.3% versus 17.4% ± 3.1% for the right (n = 24; p < 0.05). However, in organ chamber experiments, right and left IMAs exhibited comparable endothelium-dependent vasodilation to acetylcholine (n = 6). Because endothelium-derived relaxing factor induces vasodilation and also inhibits platelet adhesion, platelet aggregation, and atherogenesis, luminal release of endothelium-derived relaxing factor by the IMA could contribute to superior results when the artery is used in bypass grafting.

AB - To study the basal, luminal release of endothelium-derived relaxing factor, 35-mm segments of canine internal mammary artery (IMA) were cannulated and perfused at 5 mL/min in vitro with physiological salt solution. Vasoactive properties of the effluent were bioassayed on coronary artery smooth muscle. Effluent from IMAs produced significant vasodilation of the bioassay ring compared with effluent from a prosthetic conduit (n = 24; p < 0.05). The vasodilation by the effluent could be eliminated by mechanically removing the intima of the IMA, or by treating the IMA segments with NG-monomethyl-l-arginine or NG-nitro-l-arginine, two competitive inhibitors of nitric oxide synthesis from l-arginine; vasodilation was not influenced by treatment with indomethacin. In 83% of the superfusion experiments, effluent from the left IMA induced greater relaxation of the bioassay ring than did effluent from the right IMA. In addition, the average vasodilation induced by left IMA effluent was 28% ± 2.3% versus 17.4% ± 3.1% for the right (n = 24; p < 0.05). However, in organ chamber experiments, right and left IMAs exhibited comparable endothelium-dependent vasodilation to acetylcholine (n = 6). Because endothelium-derived relaxing factor induces vasodilation and also inhibits platelet adhesion, platelet aggregation, and atherogenesis, luminal release of endothelium-derived relaxing factor by the IMA could contribute to superior results when the artery is used in bypass grafting.

UR - http://www.scopus.com/inward/record.url?scp=0026619476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026619476&partnerID=8YFLogxK

U2 - 10.1016/0003-4975(92)90073-D

DO - 10.1016/0003-4975(92)90073-D

M3 - Article

VL - 54

SP - 1078

EP - 1084

JO - Annals of Thoracic Surgery

JF - Annals of Thoracic Surgery

SN - 0003-4975

IS - 6

ER -