Bioactive Photodegradable Hydrogel for Cultivation and Retrieval of Embryonic Stem Cells

Jungmok You, Amranul Haque, Dong Sik Shin, Kyung Jin Son, Christian Siltanen, Alexander Revzin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The development of a novel photodegradable heparin-based hydrogel for cultivation and retrieval of embryonic stem cells is described. Mouse embryonic stem cells cultured atop the gel with encapsulated growth factors (GFs) express higher levels of differentiation markers compared to a standard protocol employing soluble GFs. Beyond improving differentiation of stem cells, the novel hydrogels can be used to release specific stem cell colonies without disturbing neighboring cells. This way, stem cell colonies can be retrieved at different time points and from different locations of the culture surface for polymerase chain reaction (PCR) analysis without the loss of the microenvironment context. The ability to retrieve some stem cell colonies without disturbing neighboring colonies will open possibilities for characterizing in-dish heterogeneity of stem cell phenotype and will also allow to conserve cells/reagents. Overall, the bioactive photodegradable hydrogel developed in this study may offer new possibilities for cultivation and analysis of stem cells as well as other cell types.

Original languageEnglish (US)
Pages (from-to)4650-4656
Number of pages7
JournalAdvanced Functional Materials
Volume25
Issue number29
DOIs
StatePublished - Aug 1 2015
Externally publishedYes

Fingerprint

stem cells
Hydrogel
activity (biology)
Stem cells
Hydrogels
retrieval
Intercellular Signaling Peptides and Proteins
cells
heparins
polymerase chain reaction
phenotype
parabolic reflectors
Polymerase chain reaction
Differentiation Antigens
markers
mice
reagents
Heparin
Gels
gels

Keywords

  • embryonic stem cells
  • endoderm differentiation
  • growth factor
  • heparin
  • photodegradable gels

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Condensed Matter Physics
  • Electrochemistry

Cite this

Bioactive Photodegradable Hydrogel for Cultivation and Retrieval of Embryonic Stem Cells. / You, Jungmok; Haque, Amranul; Shin, Dong Sik; Son, Kyung Jin; Siltanen, Christian; Revzin, Alexander.

In: Advanced Functional Materials, Vol. 25, No. 29, 01.08.2015, p. 4650-4656.

Research output: Contribution to journalArticle

You, Jungmok ; Haque, Amranul ; Shin, Dong Sik ; Son, Kyung Jin ; Siltanen, Christian ; Revzin, Alexander. / Bioactive Photodegradable Hydrogel for Cultivation and Retrieval of Embryonic Stem Cells. In: Advanced Functional Materials. 2015 ; Vol. 25, No. 29. pp. 4650-4656.
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