Binding of [3H]benzo(a)pyrene metabolites to the AKR mouse embryo cell line nuclear proteins

T. H. Zytkovicz, H. L. Moses, T. C. Spelsberg

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Nuclear proteins from AKR-2B mouse embryo cells incubated 24 hr with [3H]benzo(a)pyrene (1 μg/ml; 10 μCi/ml) were purified to remove nucleic acids and characterized by isoelectric focusing and sodium dodecyl sulfate gel electrophoresis on polyacrylamide gels. Analysis of the nuclear proteins by isoelectric focusing revealed a distribution of protein in the pH gradient with two prominent bands of protein, one focusing at pH 5 and the other at pH 11, both of which bound significant amounts of the carcinogen. The acid-soluble proteins from the nuclear preparation were analyzed by isoelectric focusing, and the results also revealed two peaks of protein and radioactivity at pH's of 5 and 11. Further analysis of the acid-soluble fraction on acid urea gels revealed that significant radioactivity was associated with proteins that migrated near but slower than histone H1 which may be the high-mobility group proteins 1 and 2. Radioactivity also comigrated with histones H3 and H2B. Low levels of radioactivity were associated with histone H1 and H4.

Original languageEnglish (US)
Pages (from-to)1608-1614
Number of pages7
JournalCancer Research
Volume41
Issue number5
StatePublished - 1981

Fingerprint

Inbred AKR Mouse
Benzo(a)pyrene
Nuclear Proteins
Histones
Radioactivity
Isoelectric Focusing
Embryonic Structures
Cell Line
Acids
Polyacrylamide Gel Electrophoresis
Proteins
High Mobility Group Proteins
Proton-Motive Force
Sodium Dodecyl Sulfate
Carcinogens
Nucleic Acids
Urea
Gels

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Zytkovicz, T. H., Moses, H. L., & Spelsberg, T. C. (1981). Binding of [3H]benzo(a)pyrene metabolites to the AKR mouse embryo cell line nuclear proteins. Cancer Research, 41(5), 1608-1614.

Binding of [3H]benzo(a)pyrene metabolites to the AKR mouse embryo cell line nuclear proteins. / Zytkovicz, T. H.; Moses, H. L.; Spelsberg, T. C.

In: Cancer Research, Vol. 41, No. 5, 1981, p. 1608-1614.

Research output: Contribution to journalArticle

Zytkovicz, TH, Moses, HL & Spelsberg, TC 1981, 'Binding of [3H]benzo(a)pyrene metabolites to the AKR mouse embryo cell line nuclear proteins', Cancer Research, vol. 41, no. 5, pp. 1608-1614.
Zytkovicz, T. H. ; Moses, H. L. ; Spelsberg, T. C. / Binding of [3H]benzo(a)pyrene metabolites to the AKR mouse embryo cell line nuclear proteins. In: Cancer Research. 1981 ; Vol. 41, No. 5. pp. 1608-1614.
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