Bilirubin inhibits bile acid induced apoptosis in rat hepatocytes

A. Granato, Gregory James Gores, M. T. Vilei, R. Tolando, C. Ferraresso, M. Muraca

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Background and aims: Hydrophobic bile acids contribute to hepatocellular injury in cholestasis and rapidly induce apoptosis in vitro; however, unlike Fas agonists, cholestasis does not cause extensive hepatocyte apoptosis. As antioxidants provide protection against bile acid induced liver injury, our premise was that bilirubin, a free radical scavenger with increased plasma levels in the presence of liver disease, could protect hepatocytes against bile acid induced apoptosis. Methods: Freshly isolated rat hepatocytes were incubated for four hours with 100 μmol/l glycochenodeoxycholate (GCDC) alone or with increasing concentrations of unconjugated (UCB) or conjugated (CB) bilirubin. Results: Both UCB and CB inhibited GCDC induced apoptosis in a dose dependent fashion and suppressed the generation of reactive oxygen species by hepatocytes. Conclusions: The antiapoptotic effect of bilirubin associated with its antioxidant properties indicates that hyperbilirubinaemia may have a protective role in liver disease.

Original languageEnglish (US)
Pages (from-to)1774-1778
Number of pages5
JournalGut
Volume52
Issue number12
DOIs
StatePublished - Dec 2003

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Bile Acids and Salts
Bilirubin
Hepatocytes
Glycochenodeoxycholic Acid
Apoptosis
Cholestasis
Liver Diseases
Antioxidants
Free Radical Scavengers
Hyperbilirubinemia
Wounds and Injuries
Reactive Oxygen Species
Liver

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Granato, A., Gores, G. J., Vilei, M. T., Tolando, R., Ferraresso, C., & Muraca, M. (2003). Bilirubin inhibits bile acid induced apoptosis in rat hepatocytes. Gut, 52(12), 1774-1778. https://doi.org/10.1136/gut.52.12.1774

Bilirubin inhibits bile acid induced apoptosis in rat hepatocytes. / Granato, A.; Gores, Gregory James; Vilei, M. T.; Tolando, R.; Ferraresso, C.; Muraca, M.

In: Gut, Vol. 52, No. 12, 12.2003, p. 1774-1778.

Research output: Contribution to journalArticle

Granato, A, Gores, GJ, Vilei, MT, Tolando, R, Ferraresso, C & Muraca, M 2003, 'Bilirubin inhibits bile acid induced apoptosis in rat hepatocytes', Gut, vol. 52, no. 12, pp. 1774-1778. https://doi.org/10.1136/gut.52.12.1774
Granato A, Gores GJ, Vilei MT, Tolando R, Ferraresso C, Muraca M. Bilirubin inhibits bile acid induced apoptosis in rat hepatocytes. Gut. 2003 Dec;52(12):1774-1778. https://doi.org/10.1136/gut.52.12.1774
Granato, A. ; Gores, Gregory James ; Vilei, M. T. ; Tolando, R. ; Ferraresso, C. ; Muraca, M. / Bilirubin inhibits bile acid induced apoptosis in rat hepatocytes. In: Gut. 2003 ; Vol. 52, No. 12. pp. 1774-1778.
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