TY - JOUR
T1 - Biliary Excretion of Apolipoprotein B by the Isolated Perfused Rat Liver
T2 - Relationship to Receptor-Mediated Uptake of Human Low-Density Lipoprotein and Biliary Lipid Secretion
AU - Kawamoto, Toshio
AU - Mao, Simon J.T.
AU - Larusso, Nicholas F.
PY - 1987
Y1 - 1987
N2 - Since we previously reported that apolipoproteins, the lipid transport proteins of lipoproteins; are present in human bile, we tested the hypothesis that apolipoproteins in bile are derived from circulating lipoproteins and that they are involved in the biliary excretion of lipids. We perf used isolated rat livers with lipoprotein-free solutions and collected bile before and after addition of human low and high density lipoproteins. When human low density lipoprotein was added to solutions perf using livers isolated from rats previously treated with ethinyl estradiol, a hormone that increases receptor-mediated uptake of low density lipoproteins by hepatocytes, the concentrations in bile of cholesterol and phospholipid, but not bile acids, increased by -25% together with the appearance of small amounts (~ 1% of the amount perf used) of a metabolized form of human apolipoprotein B in bile. Acetylation of low density lipoprotein, a procedure that prevents this lipoprotein from binding to its receptor on hepatocytes, both abolished the appearance of immunoreactive apolipoprotein B in bile and blocked the increase in biliary cholesterol and phospholipid concentrations. Whereas the addition of a high density lipoprotein to solutions perf using isolated livers of normal rats was associated with the appearance of small amounts (~1% of the amount perf used) of apolipoproteins A-I and A-II in bile, there were no accompanying changes in the concentrations of biliary lipids. These results suggest that apolipoproteins or their fragments in bile are derived from circulating lipoproteins; in the case of apolipoprotein B, biliary excretion is dependent upon receptormediated uptake of low density lipoprotein by the hepatocyte. Our data are also consistent with the hypothesis that certain apolipoproteins may be involved in biliary lipid secretion, although in an as yet unclear manner.
AB - Since we previously reported that apolipoproteins, the lipid transport proteins of lipoproteins; are present in human bile, we tested the hypothesis that apolipoproteins in bile are derived from circulating lipoproteins and that they are involved in the biliary excretion of lipids. We perf used isolated rat livers with lipoprotein-free solutions and collected bile before and after addition of human low and high density lipoproteins. When human low density lipoprotein was added to solutions perf using livers isolated from rats previously treated with ethinyl estradiol, a hormone that increases receptor-mediated uptake of low density lipoproteins by hepatocytes, the concentrations in bile of cholesterol and phospholipid, but not bile acids, increased by -25% together with the appearance of small amounts (~ 1% of the amount perf used) of a metabolized form of human apolipoprotein B in bile. Acetylation of low density lipoprotein, a procedure that prevents this lipoprotein from binding to its receptor on hepatocytes, both abolished the appearance of immunoreactive apolipoprotein B in bile and blocked the increase in biliary cholesterol and phospholipid concentrations. Whereas the addition of a high density lipoprotein to solutions perf using isolated livers of normal rats was associated with the appearance of small amounts (~1% of the amount perf used) of apolipoproteins A-I and A-II in bile, there were no accompanying changes in the concentrations of biliary lipids. These results suggest that apolipoproteins or their fragments in bile are derived from circulating lipoproteins; in the case of apolipoprotein B, biliary excretion is dependent upon receptormediated uptake of low density lipoprotein by the hepatocyte. Our data are also consistent with the hypothesis that certain apolipoproteins may be involved in biliary lipid secretion, although in an as yet unclear manner.
KW - HDL
KW - LDL
KW - apo
KW - apolipoprotein
KW - high density lipopitotein
KW - low density lipoprotein
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U2 - 10.1016/S0016-5085(87)91083-3
DO - 10.1016/S0016-5085(87)91083-3
M3 - Article
C2 - 3104128
AN - SCOPUS:0023126308
SN - 0016-5085
VL - 92
SP - 1236
EP - 1242
JO - Gastroenterology
JF - Gastroenterology
IS - 5
ER -