Benzoporphyrin-lipoprotein-mediated photodestruction of intraocular tumors

Ursula Schmidt-Erfurth, Thomas J. Flotte, Evangelos S. Gragoudas, Kevin Schomacker, Reginald Birngruber, Tayyaba Hasan

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Benzoporphyrin derivative (BPD), a sensitizer currently in clinical trials, was evaluated for the treatment of experimental Greene melanoma implanted in the rabbit iris. To improve tumor targeting, BPD was complexed with low-density lipoprotein (LDL) representing an endogenous carrier system for BPD as previously described. Twelve tumors were irradiated at a sensitizer dose of 2 mg kg-1 body weight using a dye laser at 692 nm. Tumor responses were documented by photography, angiography and light and electron microscopy. All tumors treated with 80 J cm-2 regressed irreversibly. The principal mechanism of tumor necrosis was thrombosis following disruption of endothelial membranes. Ultrastructure data suggested tumor cell damage, although evidence for this being the result of direct PDT-mediated tumor cell death was less clear. These data suggest that BPD-LDL may be used to improve the selectivity of photodynamic tumor therapy possibly by the increased uptake of lipoprotein-delivered sensitizer to neovascular endothelial cells.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalExperimental Eye Research
Volume62
Issue number1
DOIs
StatePublished - Jan 1996

Keywords

  • benzoporphyrin derivative
  • in vivo
  • intraocular tumors
  • low-density lipoprotein
  • photodynamic therapy

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Benzoporphyrin-lipoprotein-mediated photodestruction of intraocular tumors'. Together they form a unique fingerprint.

  • Cite this

    Schmidt-Erfurth, U., Flotte, T. J., Gragoudas, E. S., Schomacker, K., Birngruber, R., & Hasan, T. (1996). Benzoporphyrin-lipoprotein-mediated photodestruction of intraocular tumors. Experimental Eye Research, 62(1), 1-10. https://doi.org/10.1006/exer.1996.0001