Bee venom induces apoptosis through caspase-3 activation in synovial fibroblasts of patients with rheumatoid arthritis

Seung Jae Hong, Sung Rim Gyu, In Yang Hyung, Shik Yin Chang, Gyun Koh Hyeong, Mi-Hyeon D Jang, Chang Ju Kim, Bong Keun Choe, Joo Ho Chung

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Bee venom (BV) has been used traditionally for the control of pain and inflammation in various chronic inflammatory diseases, including rheumatoid arthritis (RA) in Oriental medicine. However, it is still unclear how BV exerts its beneficial effects on the clinical course of RA patients. To investigate the effect of BV on the treatment of rheumatoid synovitis, we examined the inhibition of cell growth and induction of apoptosis in human rheumatoid synovial fibroblasts. Rheumatoid synovial fibroblasts were surgically obtained from patients with RA. Cell proliferation and viability were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptosis of synovial cells treated with 10 μg/ml BV for 24 h was identified by 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay, DNA fragmentation assay, RT-PCR, and Western blot analysis. It was demonstrated that rheumatoid synovial cells treated with 10 μg/ml BV for 24 h exhibited apoptotic features and fragmentation of DNA. In addition, BV induces apoptosis in rheumatoid synovial cells through a decrease in BCL2 expression and an increase in BAX and caspase-3 (CASP3) expression. It is suggested that BV inhibits the proliferation of rheumatoid synovial cells through induction of apoptosis by CASP3 activation.

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalToxicon
Volume46
Issue number1
DOIs
StatePublished - Jul 2005
Externally publishedYes

Fingerprint

Bee Venoms
Fibroblasts
Caspase 3
Rheumatoid Arthritis
Chemical activation
Apoptosis
Assays
DNA Fragmentation
East Asian Traditional Medicine
Synovitis
DNA Nucleotidylexotransferase
DNA
Cell proliferation
Cell growth
Labeling
Medicine
Cell Survival
Chronic Disease
Western Blotting
Cell Proliferation

Keywords

  • Apoptosis
  • Bee venom
  • Human synovial fibroblasts
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Toxicology

Cite this

Bee venom induces apoptosis through caspase-3 activation in synovial fibroblasts of patients with rheumatoid arthritis. / Hong, Seung Jae; Gyu, Sung Rim; Hyung, In Yang; Chang, Shik Yin; Hyeong, Gyun Koh; Jang, Mi-Hyeon D; Kim, Chang Ju; Choe, Bong Keun; Chung, Joo Ho.

In: Toxicon, Vol. 46, No. 1, 07.2005, p. 39-45.

Research output: Contribution to journalArticle

Hong, SJ, Gyu, SR, Hyung, IY, Chang, SY, Hyeong, GK, Jang, M-HD, Kim, CJ, Choe, BK & Chung, JH 2005, 'Bee venom induces apoptosis through caspase-3 activation in synovial fibroblasts of patients with rheumatoid arthritis', Toxicon, vol. 46, no. 1, pp. 39-45. https://doi.org/10.1016/j.toxicon.2005.03.015
Hong, Seung Jae ; Gyu, Sung Rim ; Hyung, In Yang ; Chang, Shik Yin ; Hyeong, Gyun Koh ; Jang, Mi-Hyeon D ; Kim, Chang Ju ; Choe, Bong Keun ; Chung, Joo Ho. / Bee venom induces apoptosis through caspase-3 activation in synovial fibroblasts of patients with rheumatoid arthritis. In: Toxicon. 2005 ; Vol. 46, No. 1. pp. 39-45.
@article{130b5ed6bad5483d9338450ed2ff556a,
title = "Bee venom induces apoptosis through caspase-3 activation in synovial fibroblasts of patients with rheumatoid arthritis",
abstract = "Bee venom (BV) has been used traditionally for the control of pain and inflammation in various chronic inflammatory diseases, including rheumatoid arthritis (RA) in Oriental medicine. However, it is still unclear how BV exerts its beneficial effects on the clinical course of RA patients. To investigate the effect of BV on the treatment of rheumatoid synovitis, we examined the inhibition of cell growth and induction of apoptosis in human rheumatoid synovial fibroblasts. Rheumatoid synovial fibroblasts were surgically obtained from patients with RA. Cell proliferation and viability were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptosis of synovial cells treated with 10 μg/ml BV for 24 h was identified by 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay, DNA fragmentation assay, RT-PCR, and Western blot analysis. It was demonstrated that rheumatoid synovial cells treated with 10 μg/ml BV for 24 h exhibited apoptotic features and fragmentation of DNA. In addition, BV induces apoptosis in rheumatoid synovial cells through a decrease in BCL2 expression and an increase in BAX and caspase-3 (CASP3) expression. It is suggested that BV inhibits the proliferation of rheumatoid synovial cells through induction of apoptosis by CASP3 activation.",
keywords = "Apoptosis, Bee venom, Human synovial fibroblasts, Rheumatoid arthritis",
author = "Hong, {Seung Jae} and Gyu, {Sung Rim} and Hyung, {In Yang} and Chang, {Shik Yin} and Hyeong, {Gyun Koh} and Jang, {Mi-Hyeon D} and Kim, {Chang Ju} and Choe, {Bong Keun} and Chung, {Joo Ho}",
year = "2005",
month = "7",
doi = "10.1016/j.toxicon.2005.03.015",
language = "English (US)",
volume = "46",
pages = "39--45",
journal = "Toxicon",
issn = "0041-0101",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Bee venom induces apoptosis through caspase-3 activation in synovial fibroblasts of patients with rheumatoid arthritis

AU - Hong, Seung Jae

AU - Gyu, Sung Rim

AU - Hyung, In Yang

AU - Chang, Shik Yin

AU - Hyeong, Gyun Koh

AU - Jang, Mi-Hyeon D

AU - Kim, Chang Ju

AU - Choe, Bong Keun

AU - Chung, Joo Ho

PY - 2005/7

Y1 - 2005/7

N2 - Bee venom (BV) has been used traditionally for the control of pain and inflammation in various chronic inflammatory diseases, including rheumatoid arthritis (RA) in Oriental medicine. However, it is still unclear how BV exerts its beneficial effects on the clinical course of RA patients. To investigate the effect of BV on the treatment of rheumatoid synovitis, we examined the inhibition of cell growth and induction of apoptosis in human rheumatoid synovial fibroblasts. Rheumatoid synovial fibroblasts were surgically obtained from patients with RA. Cell proliferation and viability were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptosis of synovial cells treated with 10 μg/ml BV for 24 h was identified by 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay, DNA fragmentation assay, RT-PCR, and Western blot analysis. It was demonstrated that rheumatoid synovial cells treated with 10 μg/ml BV for 24 h exhibited apoptotic features and fragmentation of DNA. In addition, BV induces apoptosis in rheumatoid synovial cells through a decrease in BCL2 expression and an increase in BAX and caspase-3 (CASP3) expression. It is suggested that BV inhibits the proliferation of rheumatoid synovial cells through induction of apoptosis by CASP3 activation.

AB - Bee venom (BV) has been used traditionally for the control of pain and inflammation in various chronic inflammatory diseases, including rheumatoid arthritis (RA) in Oriental medicine. However, it is still unclear how BV exerts its beneficial effects on the clinical course of RA patients. To investigate the effect of BV on the treatment of rheumatoid synovitis, we examined the inhibition of cell growth and induction of apoptosis in human rheumatoid synovial fibroblasts. Rheumatoid synovial fibroblasts were surgically obtained from patients with RA. Cell proliferation and viability were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptosis of synovial cells treated with 10 μg/ml BV for 24 h was identified by 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay, DNA fragmentation assay, RT-PCR, and Western blot analysis. It was demonstrated that rheumatoid synovial cells treated with 10 μg/ml BV for 24 h exhibited apoptotic features and fragmentation of DNA. In addition, BV induces apoptosis in rheumatoid synovial cells through a decrease in BCL2 expression and an increase in BAX and caspase-3 (CASP3) expression. It is suggested that BV inhibits the proliferation of rheumatoid synovial cells through induction of apoptosis by CASP3 activation.

KW - Apoptosis

KW - Bee venom

KW - Human synovial fibroblasts

KW - Rheumatoid arthritis

UR - http://www.scopus.com/inward/record.url?scp=20444497923&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444497923&partnerID=8YFLogxK

U2 - 10.1016/j.toxicon.2005.03.015

DO - 10.1016/j.toxicon.2005.03.015

M3 - Article

VL - 46

SP - 39

EP - 45

JO - Toxicon

JF - Toxicon

SN - 0041-0101

IS - 1

ER -