Abstract
To investigate whether bee venom (BV) induces apoptosis, the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay, 4,6-diamidino-2-phenylindole staining, flow cytometric analysis, and DNA fragmentation assay were performed on NCI-H1299 lung cancer cells treated with BV. Through morphological and biochemical analyses, it was demonstrated that NCI-H1299 cells treated with BV exhibit several features of apoptosis. In addition, reverse transcription-polymerase chain reaction and prostaglandin E2 (PGE2) immunoassay were performed to verify whether BV possesses an inhibitory effect on the expression of cyclooxygenase (COX) and PGE2 synthesis. Expression of COX-2 mRNA and synthesis of PGE2 were inhibited by BV. These results suggest the possibility that BV may exert an anti-tumor effect on human lung cancer.
Original language | English (US) |
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Pages (from-to) | 95-104 |
Number of pages | 10 |
Journal | Journal Pharmacological Sciences |
Volume | 91 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1 2003 |
Keywords
- Apoptosis
- Bee venom
- Cyclooxygenase
- Lung cancer
- Prostaglandin E
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology