Bee venom induces apoptosis and inhibits expression of cyclooxygenase-2 mRNA in human lung cancer cell line NCI-H1299

Mi Hyeon Jang, Min Chul Shin, Sabina Lim, Seung Moo Han, Hi Joon Park, Insop Shin, Ji Suk Lee, Kyoung Ah Kim, Ee Hwa Kim, Chang Ju Kim

Research output: Contribution to journalArticle

103 Scopus citations

Abstract

To investigate whether bee venom (BV) induces apoptosis, the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay, 4,6-diamidino-2-phenylindole staining, flow cytometric analysis, and DNA fragmentation assay were performed on NCI-H1299 lung cancer cells treated with BV. Through morphological and biochemical analyses, it was demonstrated that NCI-H1299 cells treated with BV exhibit several features of apoptosis. In addition, reverse transcription-polymerase chain reaction and prostaglandin E2 (PGE2) immunoassay were performed to verify whether BV possesses an inhibitory effect on the expression of cyclooxygenase (COX) and PGE2 synthesis. Expression of COX-2 mRNA and synthesis of PGE2 were inhibited by BV. These results suggest the possibility that BV may exert an anti-tumor effect on human lung cancer.

Original languageEnglish (US)
Pages (from-to)95-104
Number of pages10
JournalJournal Pharmacological Sciences
Volume91
Issue number2
DOIs
StatePublished - Feb 1 2003

Keywords

  • Apoptosis
  • Bee venom
  • Cyclooxygenase
  • Lung cancer
  • Prostaglandin E

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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    Jang, M. H., Shin, M. C., Lim, S., Han, S. M., Park, H. J., Shin, I., Lee, J. S., Kim, K. A., Kim, E. H., & Kim, C. J. (2003). Bee venom induces apoptosis and inhibits expression of cyclooxygenase-2 mRNA in human lung cancer cell line NCI-H1299. Journal Pharmacological Sciences, 91(2), 95-104. https://doi.org/10.1254/jphs.91.95