Bean mild mosaic virus: Genome and its translation products

V. Hari, I. P.S. Gadh, D. Baunoch, P. Das

Research output: Contribution to journalArticlepeer-review

Abstract

Viral RNA from purified preparations of bean mild mosaic virus (BMMV) was resolved into two RNAs of mol. wt 1.5×106 daltons (R1) and 0.76×106 daltons (R2). Complementary DNA hybridizations, infectivity tests, and in-vitro translation studies established that R2 is a subgenomic RNA derived from R1. In-vitro translation studies showed that the genomic RNA (R1) coded for two major proteins of mol. wt. 40.5 kD and 25.7 kD. Of these, the 40.5 kD protein was shown to be the capsid protein that is translated in vitro from both the R1 and R2 RNAs. Time-course studies on the appearance of in-vitro translation products showed that the 25.7 kD product is the earliest product made when the genomic R1 RNA is translated. This is not one of the products of R2 RNA. Several of the characteristics of BMMV described in this paper show similarities with turnip crinkle virus (TCV) and carnation mottle virus (CarMV).

Original languageEnglish (US)
Pages (from-to)211-221
Number of pages11
JournalVirus Genes
Volume2
Issue number3
DOIs
StatePublished - May 1 1989

Keywords

  • BMMV
  • C-DNA
  • Northern blot
  • bean mild mosaic virus
  • in-vitro translation
  • subgenomic RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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