Bean mild mosaic virus: Genome and its translation products

V. Hari; I. P.S. Gadh; D. Baunoch; P. Das

doi:10.1007/BF00125339

Bean mild mosaic virus: Genome and its translation products

V. Hari, I. P.S. Gadh, D. Baunoch, P. Das

Neuroscience

Research output: Contribution to journal › Article › peer-review

Abstract

Viral RNA from purified preparations of bean mild mosaic virus (BMMV) was resolved into two RNAs of mol. wt 1.5×10⁶ daltons (R1) and 0.76×10⁶ daltons (R2). Complementary DNA hybridizations, infectivity tests, and in-vitro translation studies established that R2 is a subgenomic RNA derived from R1. In-vitro translation studies showed that the genomic RNA (R1) coded for two major proteins of mol. wt. 40.5 kD and 25.7 kD. Of these, the 40.5 kD protein was shown to be the capsid protein that is translated in vitro from both the R1 and R2 RNAs. Time-course studies on the appearance of in-vitro translation products showed that the 25.7 kD product is the earliest product made when the genomic R1 RNA is translated. This is not one of the products of R2 RNA. Several of the characteristics of BMMV described in this paper show similarities with turnip crinkle virus (TCV) and carnation mottle virus (CarMV).

Original language	English (US)
Pages (from-to)	211-221
Number of pages	11
Journal	Virus Genes
Volume	2
Issue number	3
DOIs	https://doi.org/10.1007/BF00125339
State	Published - May 1989

Keywords

BMMV
C-DNA
Northern blot
bean mild mosaic virus
in-vitro translation
subgenomic RNA

ASJC Scopus subject areas

Molecular Biology
Genetics
Virology

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10.1007/BF00125339

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title = "Bean mild mosaic virus: Genome and its translation products",

abstract = "Viral RNA from purified preparations of bean mild mosaic virus (BMMV) was resolved into two RNAs of mol. wt 1.5×106 daltons (R1) and 0.76×106 daltons (R2). Complementary DNA hybridizations, infectivity tests, and in-vitro translation studies established that R2 is a subgenomic RNA derived from R1. In-vitro translation studies showed that the genomic RNA (R1) coded for two major proteins of mol. wt. 40.5 kD and 25.7 kD. Of these, the 40.5 kD protein was shown to be the capsid protein that is translated in vitro from both the R1 and R2 RNAs. Time-course studies on the appearance of in-vitro translation products showed that the 25.7 kD product is the earliest product made when the genomic R1 RNA is translated. This is not one of the products of R2 RNA. Several of the characteristics of BMMV described in this paper show similarities with turnip crinkle virus (TCV) and carnation mottle virus (CarMV).",

keywords = "BMMV, C-DNA, Northern blot, bean mild mosaic virus, in-vitro translation, subgenomic RNA",

author = "V. Hari and Gadh, {I. P.S.} and D. Baunoch and P. Das",

year = "1989",

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doi = "10.1007/BF00125339",

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T2 - Genome and its translation products

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AU - Gadh, I. P.S.

AU - Baunoch, D.

AU - Das, P.

PY - 1989/5

Y1 - 1989/5

N2 - Viral RNA from purified preparations of bean mild mosaic virus (BMMV) was resolved into two RNAs of mol. wt 1.5×106 daltons (R1) and 0.76×106 daltons (R2). Complementary DNA hybridizations, infectivity tests, and in-vitro translation studies established that R2 is a subgenomic RNA derived from R1. In-vitro translation studies showed that the genomic RNA (R1) coded for two major proteins of mol. wt. 40.5 kD and 25.7 kD. Of these, the 40.5 kD protein was shown to be the capsid protein that is translated in vitro from both the R1 and R2 RNAs. Time-course studies on the appearance of in-vitro translation products showed that the 25.7 kD product is the earliest product made when the genomic R1 RNA is translated. This is not one of the products of R2 RNA. Several of the characteristics of BMMV described in this paper show similarities with turnip crinkle virus (TCV) and carnation mottle virus (CarMV).

AB - Viral RNA from purified preparations of bean mild mosaic virus (BMMV) was resolved into two RNAs of mol. wt 1.5×106 daltons (R1) and 0.76×106 daltons (R2). Complementary DNA hybridizations, infectivity tests, and in-vitro translation studies established that R2 is a subgenomic RNA derived from R1. In-vitro translation studies showed that the genomic RNA (R1) coded for two major proteins of mol. wt. 40.5 kD and 25.7 kD. Of these, the 40.5 kD protein was shown to be the capsid protein that is translated in vitro from both the R1 and R2 RNAs. Time-course studies on the appearance of in-vitro translation products showed that the 25.7 kD product is the earliest product made when the genomic R1 RNA is translated. This is not one of the products of R2 RNA. Several of the characteristics of BMMV described in this paper show similarities with turnip crinkle virus (TCV) and carnation mottle virus (CarMV).

KW - BMMV

KW - C-DNA

KW - Northern blot

KW - bean mild mosaic virus

KW - in-vitro translation

KW - subgenomic RNA

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Bean mild mosaic virus: Genome and its translation products

Abstract

Keywords

ASJC Scopus subject areas

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