Baseline gastrointestinal eosinophilia is common in oral immunotherapy subjects with IgE-mediated peanut allergy

Benjamin L. Wright, Nielsen Q. Fernandez-Becker, Neeraja Kambham, Natasha Purington, Dana Tupa, Wenming Zhang, Matthew A. Rank, Hirohito Kita, Kelly P. Shim, Bryan J. Bunning, Alfred D. Doyle, Elizabeth A. Jacobsen, Scott D. Boyd, Mindy Tsai, Holden Maecker, Monali Manohar, Stephen J. Galli, Kari C. Nadeau, R. Sharon Chinthrajah

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Rationale: Oral immunotherapy (OIT) is an emerging treatment for food allergy. While desensitization is achieved in most subjects, many experience gastrointestinal symptoms and few develop eosinophilic gastrointestinal disease. It is unclear whether these subjects have subclinical gastrointestinal eosinophilia (GE) at baseline. We aimed to evaluate the presence of GE in subjects with food allergy before peanut OIT. Methods: We performed baseline esophagogastroduodenoscopies on 21 adults before undergoing peanut OIT. Subjects completed a detailed gastrointestinal symptom questionnaire. Endoscopic findings were assessed using the Eosinophilic Esophagitis (EoE) Endoscopic Reference Score (EREFS) and biopsies were obtained from the esophagus, gastric antrum, and duodenum. Esophageal biopsies were evaluated using the EoE Histologic Scoring System. Immunohistochemical staining for eosinophil peroxidase (EPX) was also performed. Hematoxylin and eosin and EPX stains of each biopsy were assessed for eosinophil density and EPX/mm2 was quantified using automated image analysis. Results: All subjects were asymptomatic. Pre-existing esophageal eosinophilia (>5 eosinophils per high-power field [eos/hpf]) was present in five participants (24%), three (14%) of whom had >15 eos/hpf associated with mild endoscopic findings (edema, linear furrowing, or rings; median EREFS = 0, IQR 0-0.25). Some subjects also demonstrated basal cell hyperplasia, dilated intercellular spaces, and lamina propria fibrosis. Increased eosinophils were noted in the gastric antrum (>12 eos/hpf) or duodenum (>26 eos/hpf) in 9 subjects (43%). EPX/mm2 correlated strongly with eosinophil counts (r = 0.71, p < 0.0001). Conclusions: Pre-existing GE is common in adults with IgE-mediated peanut allergy. Eosinophilic inflammation (EI) in these subjects may be accompanied by mild endoscopic and histologic findings. Longitudinal data collection during OIT is ongoing.

Original languageEnglish (US)
Article number2624
JournalFrontiers in immunology
Volume9
Issue numberNOV
DOIs
StatePublished - Nov 22 2018

Keywords

  • Eosinophilic Esophagitis
  • adverse event
  • biopsy
  • endoscopy
  • eosinophil
  • gastrointestinal
  • oral immunotherapy
  • peanut food allergy

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Wright, B. L., Fernandez-Becker, N. Q., Kambham, N., Purington, N., Tupa, D., Zhang, W., Rank, M. A., Kita, H., Shim, K. P., Bunning, B. J., Doyle, A. D., Jacobsen, E. A., Boyd, S. D., Tsai, M., Maecker, H., Manohar, M., Galli, S. J., Nadeau, K. C., & Chinthrajah, R. S. (2018). Baseline gastrointestinal eosinophilia is common in oral immunotherapy subjects with IgE-mediated peanut allergy. Frontiers in immunology, 9(NOV), [2624]. https://doi.org/10.3389/fimmu.2018.02624