Basal body reorientation mediated by a Ca2+-modulated contractile protein

G. I. McFadden, D. Schulze, B. Surek, Jeffrey L Salisbury, M. Melkonian

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

A rapid, Ca2+-dependent change in the angle between basal bodies (up to 180°) is associated with light-induced reversal of swimming direction (the 'photophobic' response) in a number of flagellated green algae. In isolated, detergent-extracted, reactivated flagellar apparatus complexes of Spermatozopsis similis, axonemal beat form conversion to the symmetrical/undulating flagellar pattern and basal body reorientation (from the antiparallel to the parallel configuration) are simultaneously induced at ≤10-7 M Ca2+. Basal body reorientation, however, is independent of flagellar beating since it is induced at ≥10-7 M Ca2+ when flagellar beating is inhibited (i.e., in the presence of 1μM orthovanadate in reactivation solutions; in the absence of ATP or dithiothreitol in isolation and reactivation solutions), or when axonemes are mechanically removed from flagellar apparatuses. Although frequent axonemal beat form reversals were induced by varying the Ca2+ concentration, antiparallel basal body configuration could not be restored in isolated flagellar apparatuses. Observations of the photophobic response in vivo indicate that even though the flagella resume the asymmetric, breaststroke beat form 1-2 s after photostimulation, antiparallel basal body configuration is not restored until a few minutes later. Using an antibody generated against the 20-kD Ca2+-modulated contractile protein of striated flagellar roots of Tetraselmis striata (Salisbury, J.L., A. Baron, B. Surek, and M. Melkonian, 1984, J. Cell Biol., 99:962-970), we have found the distal connecting fiber of Spermatozopsis similis to be immunoreactive by indirect immunofluorescence and immunogold electron microscopy. Electrophoretic and immunoblot analysis indicates that the antigen of S. similis flagellar apparatuses consists, like the Tetraselmis protein, of two acidic isoforms of 20 kD. We conclude that the distal basal body connecting fiber is a contractile organelle and reorients basal bodies during the phosphobic response in certain flagellated green algae.

Original languageEnglish (US)
Pages (from-to)903-912
Number of pages10
JournalJournal of Cell Biology
Volume105
Issue number2
StatePublished - 1987
Externally publishedYes

Fingerprint

Basal Bodies
Contractile Proteins
Chlorophyta
Axoneme
Vanadates
Flagella
Dithiothreitol
Indirect Fluorescent Antibody Technique
Organelles
Detergents
Electron Microscopy
Protein Isoforms
Adenosine Triphosphate
Light
Antigens
Antibodies

ASJC Scopus subject areas

  • Cell Biology

Cite this

McFadden, G. I., Schulze, D., Surek, B., Salisbury, J. L., & Melkonian, M. (1987). Basal body reorientation mediated by a Ca2+-modulated contractile protein. Journal of Cell Biology, 105(2), 903-912.

Basal body reorientation mediated by a Ca2+-modulated contractile protein. / McFadden, G. I.; Schulze, D.; Surek, B.; Salisbury, Jeffrey L; Melkonian, M.

In: Journal of Cell Biology, Vol. 105, No. 2, 1987, p. 903-912.

Research output: Contribution to journalArticle

McFadden, GI, Schulze, D, Surek, B, Salisbury, JL & Melkonian, M 1987, 'Basal body reorientation mediated by a Ca2+-modulated contractile protein', Journal of Cell Biology, vol. 105, no. 2, pp. 903-912.
McFadden, G. I. ; Schulze, D. ; Surek, B. ; Salisbury, Jeffrey L ; Melkonian, M. / Basal body reorientation mediated by a Ca2+-modulated contractile protein. In: Journal of Cell Biology. 1987 ; Vol. 105, No. 2. pp. 903-912.
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