TY - JOUR
T1 - Barbiturates Protect Retinal Cells from Hypoxia in Cell Culture
AU - Pulido, Jose S.
AU - Fukuda, Masakatsu
AU - Howe, Colleen A.
AU - Puro, Donald G.
PY - 1989/12
Y1 - 1989/12
N2 - A culture system was used to screen for drugs that can protect mammalian retinal cells from damage induced by hypoxia. Using a special incubator, cultures could be made hypoxic for defined periods. Phase contrast photomicroscopy facilitated comparison of retinal cells before hypoxia and 1 to 2 days after hypoxia. Using 2- to 3-week-old cultures, certain glutamate antagonists, anesthetics, calcium blockers, and thiopental sodium were screened for their effect in protecting cells from hypoxia. The most remarkable effect was noted with thiopental. Quantitative measurements showed a significant increase in the percent of cells surviving after exposure to hypoxia in the presence of 100 μmol/L of thiopental sodium compared with control hypoxic cultures—82% vs 59% at 48 hours. A dose-response curve demonstrated maximal effect at 50 μmol/L of thiopental sodium, with toxic effects noted at 200 μmol/L of thiopental sodium. Our results show that thiopental reduces hypoxia-induced damage to retinal cells in culture.
AB - A culture system was used to screen for drugs that can protect mammalian retinal cells from damage induced by hypoxia. Using a special incubator, cultures could be made hypoxic for defined periods. Phase contrast photomicroscopy facilitated comparison of retinal cells before hypoxia and 1 to 2 days after hypoxia. Using 2- to 3-week-old cultures, certain glutamate antagonists, anesthetics, calcium blockers, and thiopental sodium were screened for their effect in protecting cells from hypoxia. The most remarkable effect was noted with thiopental. Quantitative measurements showed a significant increase in the percent of cells surviving after exposure to hypoxia in the presence of 100 μmol/L of thiopental sodium compared with control hypoxic cultures—82% vs 59% at 48 hours. A dose-response curve demonstrated maximal effect at 50 μmol/L of thiopental sodium, with toxic effects noted at 200 μmol/L of thiopental sodium. Our results show that thiopental reduces hypoxia-induced damage to retinal cells in culture.
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U2 - 10.1001/archopht.1989.01070020891033
DO - 10.1001/archopht.1989.01070020891033
M3 - Article
C2 - 2597072
AN - SCOPUS:0024821399
SN - 0003-9950
VL - 107
SP - 1809
EP - 1812
JO - Archives of ophthalmology
JF - Archives of ophthalmology
IS - 12
ER -