BAFF expression correlates with idiopathic inflammatory myopathy disease activity measures and autoantibodies

Consuelo M. López De Padilla, Kelly T. McNallan, Cynthia Crowson, Hatice Bilgic, Richard J Bram, Molly S. Hein, Steven R Ytterberg, Shreyasee Amin, Erik J. Peterson, Emily C. Baechler, Ann M. Reed

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective. To investigate B cell survival cytokine messenger RNA (mRNA) levels as biomarkers of idiopathic inflammatory myopathies (IIM). Methods. We measured and compared mRNA levels of B cell survival cytokines by quantitative real-time polymerase chain reaction in 98 patients with IIM, 38 patients with systemic lupus erythematosus, and 21 healthy controls. The cytokines were B cell-activating factor belonging to the tumor necrosis factor family (BAFF);ΔBAFF; and a proliferation-inducing ligand (APRIL); and their receptors BAFF-R, transmembrane activator and calcium modulator and cyclophilin ligand interactor, and B cell maturation antigen (BCMA). We also identified autoantibodies, including anti-Sm, anti-RNP, anti-SSA/Ro, anti-SSB/La, anti-topoisomerase 1, anti-hystidyl-tRNA synthetase, anti-ribosomal P, and anti-chromatin. Clinical disease activity was assessed by the International Myositis Assessment and Clinical Studies core set tool. We examined correlation of mRNA with disease activity, medication use, and autoantibodies. Results. We found a positive correlation of BAFF and ΔBAFF expression with 3 disease activity measures, with ΔBAFF having a stronger correlation. Similarly, anti-SSA/Ro-52 and/or anti-SSA/Ro-60 had a strong positive correlation with mRNA levels of BAFF and ΔBAFF, and with relative ratios of BAFF/APRIL and BCMA/BAFF-R. Conclusion. These findings highlight the potential importance of BAFF, ΔBAFF, and BAFF-R in the pathogenesis of IIM, and suggest an important role in the assessment of disease activity.

Original languageEnglish (US)
Pages (from-to)294-302
Number of pages9
JournalJournal of Rheumatology
Volume40
Issue number3
DOIs
StatePublished - Mar 2013

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Myositis
Autoantibodies
B-Cell Maturation Antigen
Messenger RNA
Cytokines
Ligands
B-Cell Activation Factor Receptor
Cell Survival
B-Lymphocytes
B-Cell Activating Factor
Cyclophilins
Amino Acyl-tRNA Synthetases
Systemic Lupus Erythematosus
Chromatin
Real-Time Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Biomarkers
Calcium

Keywords

  • Autoantibodies
  • B cell activating factor
  • B cell activation factor receptor
  • B cell maturation antigen
  • Idiopathic inflammatory myopathies
  • Proliferation- inducing ligand

ASJC Scopus subject areas

  • Rheumatology
  • Immunology
  • Immunology and Allergy

Cite this

BAFF expression correlates with idiopathic inflammatory myopathy disease activity measures and autoantibodies. / López De Padilla, Consuelo M.; McNallan, Kelly T.; Crowson, Cynthia; Bilgic, Hatice; Bram, Richard J; Hein, Molly S.; Ytterberg, Steven R; Amin, Shreyasee; Peterson, Erik J.; Baechler, Emily C.; Reed, Ann M.

In: Journal of Rheumatology, Vol. 40, No. 3, 03.2013, p. 294-302.

Research output: Contribution to journalArticle

López De Padilla, Consuelo M. ; McNallan, Kelly T. ; Crowson, Cynthia ; Bilgic, Hatice ; Bram, Richard J ; Hein, Molly S. ; Ytterberg, Steven R ; Amin, Shreyasee ; Peterson, Erik J. ; Baechler, Emily C. ; Reed, Ann M. / BAFF expression correlates with idiopathic inflammatory myopathy disease activity measures and autoantibodies. In: Journal of Rheumatology. 2013 ; Vol. 40, No. 3. pp. 294-302.
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abstract = "Objective. To investigate B cell survival cytokine messenger RNA (mRNA) levels as biomarkers of idiopathic inflammatory myopathies (IIM). Methods. We measured and compared mRNA levels of B cell survival cytokines by quantitative real-time polymerase chain reaction in 98 patients with IIM, 38 patients with systemic lupus erythematosus, and 21 healthy controls. The cytokines were B cell-activating factor belonging to the tumor necrosis factor family (BAFF);ΔBAFF; and a proliferation-inducing ligand (APRIL); and their receptors BAFF-R, transmembrane activator and calcium modulator and cyclophilin ligand interactor, and B cell maturation antigen (BCMA). We also identified autoantibodies, including anti-Sm, anti-RNP, anti-SSA/Ro, anti-SSB/La, anti-topoisomerase 1, anti-hystidyl-tRNA synthetase, anti-ribosomal P, and anti-chromatin. Clinical disease activity was assessed by the International Myositis Assessment and Clinical Studies core set tool. We examined correlation of mRNA with disease activity, medication use, and autoantibodies. Results. We found a positive correlation of BAFF and ΔBAFF expression with 3 disease activity measures, with ΔBAFF having a stronger correlation. Similarly, anti-SSA/Ro-52 and/or anti-SSA/Ro-60 had a strong positive correlation with mRNA levels of BAFF and ΔBAFF, and with relative ratios of BAFF/APRIL and BCMA/BAFF-R. Conclusion. These findings highlight the potential importance of BAFF, ΔBAFF, and BAFF-R in the pathogenesis of IIM, and suggest an important role in the assessment of disease activity.",
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T1 - BAFF expression correlates with idiopathic inflammatory myopathy disease activity measures and autoantibodies

AU - López De Padilla, Consuelo M.

AU - McNallan, Kelly T.

AU - Crowson, Cynthia

AU - Bilgic, Hatice

AU - Bram, Richard J

AU - Hein, Molly S.

AU - Ytterberg, Steven R

AU - Amin, Shreyasee

AU - Peterson, Erik J.

AU - Baechler, Emily C.

AU - Reed, Ann M.

PY - 2013/3

Y1 - 2013/3

N2 - Objective. To investigate B cell survival cytokine messenger RNA (mRNA) levels as biomarkers of idiopathic inflammatory myopathies (IIM). Methods. We measured and compared mRNA levels of B cell survival cytokines by quantitative real-time polymerase chain reaction in 98 patients with IIM, 38 patients with systemic lupus erythematosus, and 21 healthy controls. The cytokines were B cell-activating factor belonging to the tumor necrosis factor family (BAFF);ΔBAFF; and a proliferation-inducing ligand (APRIL); and their receptors BAFF-R, transmembrane activator and calcium modulator and cyclophilin ligand interactor, and B cell maturation antigen (BCMA). We also identified autoantibodies, including anti-Sm, anti-RNP, anti-SSA/Ro, anti-SSB/La, anti-topoisomerase 1, anti-hystidyl-tRNA synthetase, anti-ribosomal P, and anti-chromatin. Clinical disease activity was assessed by the International Myositis Assessment and Clinical Studies core set tool. We examined correlation of mRNA with disease activity, medication use, and autoantibodies. Results. We found a positive correlation of BAFF and ΔBAFF expression with 3 disease activity measures, with ΔBAFF having a stronger correlation. Similarly, anti-SSA/Ro-52 and/or anti-SSA/Ro-60 had a strong positive correlation with mRNA levels of BAFF and ΔBAFF, and with relative ratios of BAFF/APRIL and BCMA/BAFF-R. Conclusion. These findings highlight the potential importance of BAFF, ΔBAFF, and BAFF-R in the pathogenesis of IIM, and suggest an important role in the assessment of disease activity.

AB - Objective. To investigate B cell survival cytokine messenger RNA (mRNA) levels as biomarkers of idiopathic inflammatory myopathies (IIM). Methods. We measured and compared mRNA levels of B cell survival cytokines by quantitative real-time polymerase chain reaction in 98 patients with IIM, 38 patients with systemic lupus erythematosus, and 21 healthy controls. The cytokines were B cell-activating factor belonging to the tumor necrosis factor family (BAFF);ΔBAFF; and a proliferation-inducing ligand (APRIL); and their receptors BAFF-R, transmembrane activator and calcium modulator and cyclophilin ligand interactor, and B cell maturation antigen (BCMA). We also identified autoantibodies, including anti-Sm, anti-RNP, anti-SSA/Ro, anti-SSB/La, anti-topoisomerase 1, anti-hystidyl-tRNA synthetase, anti-ribosomal P, and anti-chromatin. Clinical disease activity was assessed by the International Myositis Assessment and Clinical Studies core set tool. We examined correlation of mRNA with disease activity, medication use, and autoantibodies. Results. We found a positive correlation of BAFF and ΔBAFF expression with 3 disease activity measures, with ΔBAFF having a stronger correlation. Similarly, anti-SSA/Ro-52 and/or anti-SSA/Ro-60 had a strong positive correlation with mRNA levels of BAFF and ΔBAFF, and with relative ratios of BAFF/APRIL and BCMA/BAFF-R. Conclusion. These findings highlight the potential importance of BAFF, ΔBAFF, and BAFF-R in the pathogenesis of IIM, and suggest an important role in the assessment of disease activity.

KW - Autoantibodies

KW - B cell activating factor

KW - B cell activation factor receptor

KW - B cell maturation antigen

KW - Idiopathic inflammatory myopathies

KW - Proliferation- inducing ligand

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