Bacterial gene control by DNA looping using engineered dimeric transcription activator like effector (TALE) proteins

Nicole A. Becker, Tanya L. Schwab, Karl J. Clark, L. James Maher

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Genetic switches must alternate between states whose probabilities are dependent on regulatory signals. Classical examples of transcriptional control in bacteria depend on repressive DNA loops anchored by proteins whose structures are sensitive to small molecule inducers or co-repressors. We are interested in exploiting these natural principles to engineer artificial switches for transcriptional control of bacterial genes. Here, we implement designed homodimeric DNA looping proteins ('Transcription Activator-Like Effector Dimers'; TALEDs) for this purpose in living bacteria. Using well-studied FKBP dimerization domains, we build switches that mimic regulatory characteristics of classical Escherichia coli lactose, galactose and tryptophan operon promoters, including induction or co-repression by small molecules. Engineered DNA looping using TALEDs is thus a new approach to tuning gene expression in bacteria. Similar principles may also be applicable for gene control in eukaryotes.

Original languageEnglish (US)
Pages (from-to)2690-2696
Number of pages7
JournalNucleic acids research
Volume46
Issue number5
DOIs
StatePublished - Mar 16 2018

ASJC Scopus subject areas

  • Genetics

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