Bacterial expression of Scapharca dimeric hemoglobin: A simple model system for investigating protein cooperativity

C. M. Summerford, Animesh D Pardanani, A. H. Betts, A. R. Poteete, G. Colotti, W. E. Royer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Recombinant Scapharca homodimeric hemoglobin has been expressed at high levels from a synthetic gene in Escherichia coli. Addition of the heme precursor 6-aminolevulinic acid to the expression culture results in a considerable increase in the yield of soluble hemoglobin. The recombinant hemoglobin exhibits cooperative oxygen binding properties indistinguishable from native protein. Crystals of the recombinant protein, like those of native hemoglobin, diffract to high resolution which will allow functional studies of site-directed mutants to be correlated with detailed structural analyses.

Original languageEnglish (US)
Pages (from-to)593-599
Number of pages7
JournalProtein Engineering
Volume8
Issue number6
StatePublished - 1995
Externally publishedYes

Fingerprint

Scapharca
Hemoglobin
Hemoglobins
Proteins
Synthetic Genes
Recombinant proteins
Aminolevulinic Acid
Heme
Recombinant Proteins
Escherichia coli
Genes
Oxygen
Crystals
Acids

Keywords

  • δ-aminolevulinic acid
  • Bacterial expression
  • Cooperativity
  • Hemoglobins
  • Subunit assembly

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Bioengineering
  • Biotechnology

Cite this

Summerford, C. M., Pardanani, A. D., Betts, A. H., Poteete, A. R., Colotti, G., & Royer, W. E. (1995). Bacterial expression of Scapharca dimeric hemoglobin: A simple model system for investigating protein cooperativity. Protein Engineering, 8(6), 593-599.

Bacterial expression of Scapharca dimeric hemoglobin : A simple model system for investigating protein cooperativity. / Summerford, C. M.; Pardanani, Animesh D; Betts, A. H.; Poteete, A. R.; Colotti, G.; Royer, W. E.

In: Protein Engineering, Vol. 8, No. 6, 1995, p. 593-599.

Research output: Contribution to journalArticle

Summerford, CM, Pardanani, AD, Betts, AH, Poteete, AR, Colotti, G & Royer, WE 1995, 'Bacterial expression of Scapharca dimeric hemoglobin: A simple model system for investigating protein cooperativity', Protein Engineering, vol. 8, no. 6, pp. 593-599.
Summerford, C. M. ; Pardanani, Animesh D ; Betts, A. H. ; Poteete, A. R. ; Colotti, G. ; Royer, W. E. / Bacterial expression of Scapharca dimeric hemoglobin : A simple model system for investigating protein cooperativity. In: Protein Engineering. 1995 ; Vol. 8, No. 6. pp. 593-599.
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