B7-H1 expressed by activated CD8 T cells is essential for their survival

Vesna Pulko, Kimberley J. Harris, Xin Liu, Rachel M. Gibbons, Susan M. Harrington, Christopher J. Krco, Eugene D Kwon, Haidong M Dong

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Abstract

An immunoinhibitory role of B7 homologue 1 (B7-H1) expressed by non-T cells has been established; however, the function of B7-H1 expressed by T cells is not clear. Peak expression of B7-H1 on Ag-primed CD8 T cells was observed during the contraction phase of an immune response. Unexpectedly, B7-H1 blockade at this stage reduced the numbers of effector CD8 T cells, suggesting B7-H1 blocking Ab may disturb an unknown function of B7-H1 expressed by CD8 T cells. To exclusively examine the role of B7-H1 expressed by T cells, we introduced B7-H1 deficiency into TCR transgenic (OT-1) mice. Naive B7-H1-deficient CD8 T cells proliferated normally following Ag stimulation; however, once activated, they underwent more robust contraction in vivo and more apoptosis in vitro. In addition, B7-H1-deficient CD8 T cells were more sensitive to Ca-dependent and Fas ligand-dependent killing by cytotoxic T lymphocytes. Activation-induced Bcl-xL expression was lower in activated B7-H1-deficient CD8 T cells, whereas Bcl-2 and Bim expression were comparable to the wild type. Transfer of effector B7-H1-deficient CD8 T cells failed to suppress tumor growth in vivo. Thus, upregulation of B7-H1 on primed T cells helps effector T cells survive the contraction phase and consequently generate optimal protective immunity.

Original languageEnglish (US)
Pages (from-to)5606-5614
Number of pages9
JournalJournal of Immunology
Volume187
Issue number11
DOIs
StatePublished - Dec 1 2011

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T-Lymphocytes
Fas Ligand Protein
Cytotoxic T-Lymphocytes
Immunity
Up-Regulation
Apoptosis
Growth
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Pulko, V., Harris, K. J., Liu, X., Gibbons, R. M., Harrington, S. M., Krco, C. J., ... Dong, H. M. (2011). B7-H1 expressed by activated CD8 T cells is essential for their survival. Journal of Immunology, 187(11), 5606-5614. https://doi.org/10.4049/jimmunol.1003976

B7-H1 expressed by activated CD8 T cells is essential for their survival. / Pulko, Vesna; Harris, Kimberley J.; Liu, Xin; Gibbons, Rachel M.; Harrington, Susan M.; Krco, Christopher J.; Kwon, Eugene D; Dong, Haidong M.

In: Journal of Immunology, Vol. 187, No. 11, 01.12.2011, p. 5606-5614.

Research output: Contribution to journalArticle

Pulko, V, Harris, KJ, Liu, X, Gibbons, RM, Harrington, SM, Krco, CJ, Kwon, ED & Dong, HM 2011, 'B7-H1 expressed by activated CD8 T cells is essential for their survival', Journal of Immunology, vol. 187, no. 11, pp. 5606-5614. https://doi.org/10.4049/jimmunol.1003976
Pulko V, Harris KJ, Liu X, Gibbons RM, Harrington SM, Krco CJ et al. B7-H1 expressed by activated CD8 T cells is essential for their survival. Journal of Immunology. 2011 Dec 1;187(11):5606-5614. https://doi.org/10.4049/jimmunol.1003976
Pulko, Vesna ; Harris, Kimberley J. ; Liu, Xin ; Gibbons, Rachel M. ; Harrington, Susan M. ; Krco, Christopher J. ; Kwon, Eugene D ; Dong, Haidong M. / B7-H1 expressed by activated CD8 T cells is essential for their survival. In: Journal of Immunology. 2011 ; Vol. 187, No. 11. pp. 5606-5614.
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