B lymphopoiesis is active throughout human life, but there are developmental age-related changes

Maria Isabel D Rossi, Takafumi Yokota, Kay L Medina, Karla P. Garrett, Philip C. Comp, Arthur H. Schipul, Paul W. Kincade

Research output: Contribution to journalArticle

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Abstract

This study addressed several questions concerning age-related changes in human B lymphopoiesis. The relative abundance of pro-B, pre-B, immature, naive, and mature B cells among the CD19+ lymphocyte fraction of human bone marrow was found not to change appreciably over the interval between 24 and 88 years of age. Moreover, proliferation of pro-B and large pre-B cells in adult marrow equaled that observed with fetal marrow specimens. Exceptionally low numbers of lymphocyte precursors were found in some marrow samples, and the values obtained were used to determine parameters that best reflect B lymphopoiesis. Cord blood always contained higher incidences of functional precursors than adult cells. However, sorted CD34+ Lin- CD10+ progenitors from cord blood and adult marrow had equivalent potential for differentiation in culture, and notable age-related changes were found in more primitive subsets. A recently described subset of CD34+CD38-CD7+ cord blood cells had no exact counterpart in adult marrow. That is, all adult CD34+Lin-CD7+CD10- cells expressed CD38, displayed less CD45RA, and had little B-lineage differentiation potential. The CD7+ fractions in either site contained progenitors for erythroid and natural killer (NK) lineages, and ones sorted from marrow expressed high levels of transcripts for the CD122 interleukin 2 (IL-2)/IL-15 receptor required by NK-lineage precursors. Dramatic changes in human B lymphopoiesis occur early in life, and more information is required to construct a probable sequence of differentiation events prior to the acquisition of CD10.

Original languageEnglish (US)
Pages (from-to)576-584
Number of pages9
JournalBlood
Volume101
Issue number2
DOIs
StatePublished - Jan 15 2003
Externally publishedYes

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Lymphopoiesis
Blood
Lymphocytes
Bone Marrow
Cells
Interleukin-15 Receptors
Fetal Blood
Interleukin-2
Bone
B-Lymphoid Precursor Cells
Lymphocyte Count
Blood Cells
B-Lymphocytes
Incidence

ASJC Scopus subject areas

  • Hematology

Cite this

Rossi, M. I. D., Yokota, T., Medina, K. L., Garrett, K. P., Comp, P. C., Schipul, A. H., & Kincade, P. W. (2003). B lymphopoiesis is active throughout human life, but there are developmental age-related changes. Blood, 101(2), 576-584. https://doi.org/10.1182/blood-2002-03-0896

B lymphopoiesis is active throughout human life, but there are developmental age-related changes. / Rossi, Maria Isabel D; Yokota, Takafumi; Medina, Kay L; Garrett, Karla P.; Comp, Philip C.; Schipul, Arthur H.; Kincade, Paul W.

In: Blood, Vol. 101, No. 2, 15.01.2003, p. 576-584.

Research output: Contribution to journalArticle

Rossi, MID, Yokota, T, Medina, KL, Garrett, KP, Comp, PC, Schipul, AH & Kincade, PW 2003, 'B lymphopoiesis is active throughout human life, but there are developmental age-related changes', Blood, vol. 101, no. 2, pp. 576-584. https://doi.org/10.1182/blood-2002-03-0896
Rossi, Maria Isabel D ; Yokota, Takafumi ; Medina, Kay L ; Garrett, Karla P. ; Comp, Philip C. ; Schipul, Arthur H. ; Kincade, Paul W. / B lymphopoiesis is active throughout human life, but there are developmental age-related changes. In: Blood. 2003 ; Vol. 101, No. 2. pp. 576-584.
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