B cells are important as antigen presenting cells for induction of MHC-restricted arthritis in transgenic mice

Veena D Taneja, Christopher J. Krco, Marshall D. Behrens, Harvinder S. Luthra, Marie M. Griffiths, Chella S. David

Research output: Contribution to journalArticle

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Abstract

Rheumatoid arthritis and its animal model, collagen-induced arthritis, are known as a T and B cell dependent disease. To analyze the role of B cells in arthritis, we generated B cell deficient (μMT) mice carrying HLA-DQ8 as transgene, Aβo.DQ8.μmt mice. HLA-DQ8 transgenic mice (Aβo.DQ8) are susceptible to collagen induced arthritis, an animal model for inflammatory arthritis. Deletion of IgM gene led to the absence of B cells while T cells were comparable to Aβo.DQ8 mice. Arthritis and autoantibodies was completely abrogated in B cell deficient DQ8 mice. T cell response and proinflammatory cytokine production in response to type II collagen and its derived peptides in vitro was significantly decreased despite an increased number of Mac-1 positive cells in DQ8.μmt mice compared to DQ8 mice suggesting B cells could be important for antigen presentation as well. In vitro substitution of B cells from wild type mice restored the response in DQ8.μmt mice. B cells could also present CII-derived peptides to antigen-specific DQ8-restricted hybridomas reinforcing the role of B cells in presentation of antigens to T cells. The data suggest that B cells can be involved in pathogenesis of arthritis by producing autoantibodies and antigen presentation.

Original languageEnglish (US)
Pages (from-to)2988-2996
Number of pages9
JournalMolecular Immunology
Volume44
Issue number11
DOIs
StatePublished - Apr 2007

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Antigen-Presenting Cells
Transgenic Mice
Arthritis
B-Lymphocytes
Antigen Presentation
T-Lymphocytes
Experimental Arthritis
Autoantibodies
Animal Models
Peptides
Collagen Type II
Gene Deletion
Hybridomas
Transgenes
Immunoglobulin M
Rheumatoid Arthritis
Cytokines
Antigens

Keywords

  • Antigen presentation
  • Autoantibodies
  • B cells
  • Rheumatoid arthritis
  • Transgenic/knockout mice

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

B cells are important as antigen presenting cells for induction of MHC-restricted arthritis in transgenic mice. / Taneja, Veena D; Krco, Christopher J.; Behrens, Marshall D.; Luthra, Harvinder S.; Griffiths, Marie M.; David, Chella S.

In: Molecular Immunology, Vol. 44, No. 11, 04.2007, p. 2988-2996.

Research output: Contribution to journalArticle

Taneja, Veena D ; Krco, Christopher J. ; Behrens, Marshall D. ; Luthra, Harvinder S. ; Griffiths, Marie M. ; David, Chella S. / B cells are important as antigen presenting cells for induction of MHC-restricted arthritis in transgenic mice. In: Molecular Immunology. 2007 ; Vol. 44, No. 11. pp. 2988-2996.
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